ID   FTHS_CLOK1              Reviewed;         558 AA.
AC   B9DYW1;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=CKR_0385;
OS   Clostridium kluyveri (strain NBRC 12016).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=583346;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 12016;
RA   Inui M., Nonaka H., Shinoda Y., Ikenaga Y., Abe M., Naito K., Vertes A.A.,
RA   Yukawa H.;
RT   "Complete genome sequence of Clostridium kluyveri and comparative genomics
RT   of Clostridia species.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AP009049; BAH05436.1; -; Genomic_DNA.
DR   RefSeq; WP_011989009.1; NC_011837.1.
DR   AlphaFoldDB; B9DYW1; -.
DR   SMR; B9DYW1; -.
DR   EnsemblBacteria; BAH05436; BAH05436; CKR_0385.
DR   KEGG; ckr:CKR_0385; -.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000007969; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..558
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000185253"
FT   BINDING         66..73
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   558 AA;  60414 MW;  6E7ABE3A88C40AE8 CRC64;
     MNFKSDIQIA QECSMENIKD IAKKLNIFED EIELYGKYKA KIDYNLLKTT KGKNGKLILC
     TAINPTPAGE GKTTTSIGVA DALARLDKSV VVALREPSMG PVFGIKGGAA GGGYAQVVPM
     EDINLHFTGD IHAMTAANNL LAALIDNHIY QGNKLNIDSR RVVWRRCVDM NDRQLRFVVD
     GLGGKVNGIP REDGFDITVA SEIMAIFCLS TDINDLKERI SKIVVGYTTE GNPVTAHDLK
     AEGAMAALLK DALKPNLVQT LEGTPAFVHG GPFANIAHGC NSIMATRMAM HFGDYVVTEA
     GFGADLGAEK FLDIKCRMAG LRPDAVIIVA TVRALKYNGG TPKTKLNNEN LETLEKGIPN
     LLKHVENITK VFKLPAVVAL NAFPTDTEAE LKLVEEKCRE FGVSVKLSEV WAKGGEGGIE
     VAKEVLRLIN EGKNDFQFAY DEKLPIRDKI RAIAQKIYGA DGVTFTNQAE KEIDELEKLG
     FGKTPVCIAK TQYSLTDDQN KLGRPKGFKI TVRQVSISAG AGFVVAITGS IMKMPGLPKV
     PAAEKIDVDE NGVISGLF