ID   FTHS_DEHM1              Reviewed;         597 AA.
AC   Q3Z8K3;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs1 {ECO:0000255|HAMAP-Rule:MF_01543}; Synonyms=fhs-1;
GN   OrderedLocusNames=DET0671;
GN   and
GN   Name=fhs2 {ECO:0000255|HAMAP-Rule:MF_01543}; Synonyms=fhs-2;
GN   OrderedLocusNames=DET0705;
OS   Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195)
OS   (Dehalococcoides ethenogenes (strain 195)).
OC   Bacteria; Chloroflexi; Dehalococcoidia; Dehalococcoidales;
OC   Dehalococcoidaceae; Dehalococcoides.
OX   NCBI_TaxID=243164;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-2266 / KCTC 15142 / 195;
RX   PubMed=15637277; DOI=10.1126/science.1102226;
RA   Seshadri R., Adrian L., Fouts D.E., Eisen J.A., Phillippy A.M., Methe B.A.,
RA   Ward N.L., Nelson W.C., DeBoy R.T., Khouri H.M., Kolonay J.F., Dodson R.J.,
RA   Daugherty S.C., Brinkac L.M., Sullivan S.A., Madupu R., Nelson K.E.,
RA   Kang K.H., Impraim M., Tran K., Robinson J.M., Forberger H.A., Fraser C.M.,
RA   Zinder S.H., Heidelberg J.F.;
RT   "Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides
RT   ethenogenes.";
RL   Science 307:105-108(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000027; AAW39992.1; -; Genomic_DNA.
DR   EMBL; CP000027; AAW40064.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q3Z8K3; -.
DR   SMR; Q3Z8K3; -.
DR   STRING; 243164.DET0671; -.
DR   EnsemblBacteria; AAW39992; AAW39992; DET0705.
DR   EnsemblBacteria; AAW40064; AAW40064; DET0671.
DR   KEGG; det:DET0671; -.
DR   KEGG; det:DET0705; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_0; -.
DR   OMA; CGEIMTM; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000008289; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..597
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199344"
FT   BINDING         84..91
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   597 AA;  64225 MW;  87DB9B994C1B9E89 CRC64;
     MTINNRKSHL KDLDPGLMKD WEIAEKAEEF LKPSKMLAEE LGLTEDEIIP HGKYVAKVDF
     AGVLTRLKDR PNGKYIDVTA ITPTPLGEGK STTTMGLVQG LGNLGKKVTG AIRQPSSGPT
     FNIKGSAAGG GRSQCLPLSP FTLGLTGDID AVTNSHNLAM VALQARIQHE ANNTDEFLSS
     RSLKRLDIDP ARVELKWAID FCAQSLREII MGIGGKTDGY QMHSGFGISV SSEVMAILSV
     FTGLADLRER MSKIIVAYRQ NGEPVTTADL EVDGAMTALL LRAVNPNLLQ TIEGQPVFVH
     AGPFANIAIG QSSIVADRLA LKLADYHVTE SGFGADIGFE KFWNIKCRLS GLKPDCAVIV
     ATVRALKMHG GGPKVTPGAP LDPAYTTPNA ALVEKGCQNM LAHIQTVKTA GINPVVCINH
     FAADTAQEID IIRRTAEQAG ARVAVSYHWA NGGEGAAELA EAVIDACNEP NDFHFLYPED
     MPLRERIYTI ARKVYGADGV SYTQTALEKL ARLENTGNTQ FMPSCMVKTH LSLSHDPALK
     GRPGGFTLPI RDILTYMGAG LVVPVAGDIK LMPGTSSDPN FRRIDIDTHT GKVKGLF