FTHS_DEHMC
ID FTHS_DEHMC Reviewed; 597 AA.
AC Q3ZX40;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=cbdbA660;
OS Dehalococcoides mccartyi (strain CBDB1).
OC Bacteria; Chloroflexi; Dehalococcoidia; Dehalococcoidales;
OC Dehalococcoidaceae; Dehalococcoides.
OX NCBI_TaxID=255470;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBDB1;
RX PubMed=16116419; DOI=10.1038/nbt1131;
RA Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.;
RT "Genome sequence of the chlorinated compound-respiring bacterium
RT Dehalococcoides species strain CBDB1.";
RL Nat. Biotechnol. 23:1269-1273(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; AJ965256; CAI82827.1; -; Genomic_DNA.
DR AlphaFoldDB; Q3ZX40; -.
DR SMR; Q3ZX40; -.
DR KEGG; deh:cbdbA660; -.
DR HOGENOM; CLU_003601_3_3_0; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..597
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199345"
FT BINDING 84..91
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 597 AA; 64493 MW; 775FDB2876B52CB5 CRC64;
MAVNDREFHL KELDPVLMKD WEIAEKAEDF LKPVKVLAME LGLTEDEIIP HGKYIAKVDF
AGVLARLKDR PNGKYIDVTA ITPTPLGEGK STTTMGLVQG LGKLGKKVTG AIRQPSSGPT
FNIKGSAAGG GLSQCLPLSP FSLGLTGDID AVTNSHNLAM VALQARLQHE ANNTDEFLSS
RHLTRLDIDP SRVEMKWAMD FCAQSLREII MGIGGKTDGY RMPSGFGISV SSEVMAILSV
FTSLSDLRER MGKIIVAYRK NDEPVTTADL EVDGAMTALL LRAVNPNLLQ TIEGQPVFVH
AGPFANIAIG QSSIVADRLA LKLADYHVTE SGFGADIGFE KFWNIKCRLS GLKPDCAVIV
VTARALKMHG GGPKVTPGAP LDPAYTTPNT KLVEKGCQNM LAHIQTVKTA GINPVVCINH
FAADTHEEID IIRRTAEQAE ARVAVSHHWA KGGDGATELA EAVTDACNEP NNFHFLYPEN
MPLKKRIETI ARKVYGANGV SYTPIAMEKL ARIESMGDTQ FMPTCMVKTH LSLSHDPALK
GRPSGFTLPI RDILTYMGAG LVVPVAGDIK LMPGTSSDPN FKRIDVDTQT GKVKGLF
