FTHS_DESAL
ID FTHS_DESAL Reviewed; 587 AA.
AC B8FGU7;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Dalk_3639;
OS Desulfatibacillum aliphaticivorans.
OC Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales;
OC Desulfobacteraceae; Desulfatibacillum.
OX NCBI_TaxID=218208;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AK-01;
RX PubMed=21651686; DOI=10.1111/j.1462-2920.2011.02516.x;
RA Callaghan A.V., Morris B.E., Pereira I.A., McInerney M.J., Austin R.N.,
RA Groves J.T., Kukor J.J., Suflita J.M., Young L.Y., Zylstra G.J., Wawrik B.;
RT "The genome sequence of Desulfatibacillum alkenivorans AK-01: a blueprint
RT for anaerobic alkane oxidation.";
RL Environ. Microbiol. 14:101-113(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP001322; ACL05327.1; -; Genomic_DNA.
DR RefSeq; WP_015948384.1; NC_011768.1.
DR AlphaFoldDB; B8FGU7; -.
DR SMR; B8FGU7; -.
DR EnsemblBacteria; ACL05327; ACL05327; Dalk_3639.
DR KEGG; dal:Dalk_3639; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_7; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000739; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..587
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000146680"
FT BINDING 73..80
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 587 AA; 63626 MW; 1C06F76B2D27B021 CRC64;
MAYNAVEMAD WQISEAAEEN MPTPEQWAEK LGLQSDEMLP MGRLAKVDFL KIIDRLKDRP
DGKYIEVTAI TPTPLGEGKS TTSVGLMEGL GMRGKNVGGA LRQPSGGPTM NVKGTAAGGG
NSLLIPMTEF SLGLTGDIND IMNAHNLGMV AMTARMQHER NYNDEQLQRL TGMRRLDIDP
TRVEFGWIID FCAQALRNIV IGLGGRFDGY TMQSKFGIAV GSELMAILAV ATDLADLKER
INNITVAFDK SGKPVTCGDL EVGNAMAAFM RNTINPTLMC TAEYQPCFVH AGPFANIAVG
QSSIIADRVG LKLFDYHVTE SGFAADIGFE KFWNVKCRFS GLKPHVSVLT STIRALKMHG
GGPKVVAGKA LDDAYTKENL ELVEKGCENM VHMIGVIRKA GINPVVCINR FYTDTDAECA
IVRKAAEAAG ARCAESKHWE KGGEGALEFA DAVIEACEEG NDFDFLYPLE MKLRDRVNKI
ATEVYGADGV DWAPEAAAKA EMLESDPKYA DFATMMVKTH LSLSHDPVKK GVPKGWRLPI
RDVLIYSGAK FLCPCAGTIS LMPGTGSNPA FRRIDVDPAT GKVSGLF
