ID   FTHS_FERNB              Reviewed;         552 AA.
AC   A7HLZ4;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Fnod_1079;
OS   Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1).
OC   Bacteria; Thermotogae; Thermotogales; Fervidobacteriaceae;
OC   Fervidobacterium.
OX   NCBI_TaxID=381764;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35602 / DSM 5306 / Rt17-B1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C.,
RA   Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.;
RT   "Complete sequence of Fervidobacterium nodosum Rt17-B1.";
RL   Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000771; ABS60927.1; -; Genomic_DNA.
DR   RefSeq; WP_011994240.1; NC_009718.1.
DR   AlphaFoldDB; A7HLZ4; -.
DR   SMR; A7HLZ4; -.
DR   STRING; 381764.Fnod_1079; -.
DR   PRIDE; A7HLZ4; -.
DR   EnsemblBacteria; ABS60927; ABS60927; Fnod_1079.
DR   KEGG; fno:Fnod_1079; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_0; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000002415; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..552
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000073555"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   552 AA;  59243 MW;  C92C5736F192B44D CRC64;
     MLSDIEIAKQ ARLKDIRDIA KFLDIPEDML KPYGRYIAKV DHRYLNTLNA KPNGKLILVT
     AITPTPAGEG KTTTSIGLSM ALNRIGKKSI VTLREPSLGP VFGVKGGAAG GGYSQVLPME
     DINLHFTGDI HAVTTAHNLI AAMIDAHINH GNELGIDLRK IYWKRAMDMN DRALREIVIA
     LGGSANGYPR EDGFLITAAS EIMAVLCLAK DLTDLKRRIG EIVIAQGKNG LVRVKDIQAE
     GAAAALLKDA INPNLVQTIE NTPAFVHGGP FANIAHGTNT LIATKLALKL SDYVVTEAGF
     AADLGAQKFL DFVAPTGGLF VDAVVIVASI RAMKYHGGVS KDNLNEENVD AVIKGLENLK
     VHIENMKKYG VPVVVALNRF STDTEKEIEA VLKNSPAKCV LNEAYAKGSE GAIELAKAVV
     ETIENVPSNY KPLVPSDLPV EQKIELIAKE IYRAGKVIYT DTAKSKLSML KKNGFGNYPV
     IIAKTQNSIS DDPKKINAPS GYEFTVRDFQ ISAGAGFVVA LAGEIMLMPG LPKAPAAVNI
     DIDENGEITG LF