ID   FTHS_FUSNN              Reviewed;         544 AA.
AC   Q8RHF4;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=FN2082;
OS   Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 /
OS   BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355).
OC   Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium.
OX   NCBI_TaxID=190304;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC
RC   2640 / LMG 13131 / VPI 4355;
RX   PubMed=11889109; DOI=10.1128/jb.184.7.2005-2018.2002;
RA   Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A.,
RA   Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O.,
RA   Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N., D'Souza M.,
RA   Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C.,
RA   Overbeek R.;
RT   "Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum
RT   strain ATCC 25586.";
RL   J. Bacteriol. 184:2005-2018(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AE009951; AAL94166.1; -; Genomic_DNA.
DR   RefSeq; NP_602867.1; NC_003454.1.
DR   AlphaFoldDB; Q8RHF4; -.
DR   SMR; Q8RHF4; -.
DR   STRING; 190304.FN2082; -.
DR   PRIDE; Q8RHF4; -.
DR   EnsemblBacteria; AAL94166; AAL94166; FN2082.
DR   KEGG; fnu:FN2082; -.
DR   PATRIC; fig|190304.8.peg.544; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_0; -.
DR   InParanoid; Q8RHF4; -.
DR   OMA; CGEIMTM; -.
DR   BioCyc; FNUC190304:G1FZS-567-MON; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000002521; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..544
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199349"
FT   BINDING         63..70
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   544 AA;  58321 MW;  C3CAEF3D15625B75 CRC64;
     MTDIQIAQAA KKENIVEIAK KLGLTEDDIE QYGKYKAKVN LDVLQKNKRP NGKLILVTAI
     TPTPAGEGKS TVTIGLTQAL NKMGKLSAAA IREPSLGPVF GMKGGAAGGG YAQVVPMEDI
     NLHFTGDMHA IGIAHNLISA CIDNHINSGN ALGIDVTKIT WKRVVDMNDR ALRNIVIGLG
     GKANGYPRQD SFQITVGSEI MAILCLSNSI TELKEKIKNI VIGTSVTGKL IKVGDFHIEG
     AVAALLKDAI KPNLVQTLEN TPVFIHGGPF ANIAHGCNSI LATKMALKLT DYVVTEAGFA
     ADLGAEKFID IKCRLGGLKP DCAVIVATVR ALEHHGKGDL KAGLENLDKH IDNIKNKYKL
     PLVVAINKFI TDTDEQINMI EKFCNERGAE VSLCEVWAKG GEGGIDLAEK VLKAIDNNKT
     EFDYFYDINL TIKEKIEKIC KEIYGADGVI FAPATKKVFD VIEAEGLNKL PVCMSKTQKS
     ISDNPALLGK PTGFKVTIND LRLAVGAGFV IAMAGDIIDM PGLPKKPSAE VIDIDENGVI
     SGLF