FTHS_HALOH
ID FTHS_HALOH Reviewed; 554 AA.
AC B8D067;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Hore_00600;
OS Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562).
OC Bacteria; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae;
OC Halothermothrix.
OX NCBI_TaxID=373903;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H 168 / OCM 544 / DSM 9562;
RX PubMed=19145256; DOI=10.1371/journal.pone.0004192;
RA Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H.,
RA Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.;
RT "Genome analysis of the anaerobic thermohalophilic bacterium
RT Halothermothrix orenii.";
RL PLoS ONE 4:E4192-E4192(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP001098; ACL68821.1; -; Genomic_DNA.
DR RefSeq; WP_012635020.1; NC_011899.1.
DR AlphaFoldDB; B8D067; -.
DR SMR; B8D067; -.
DR STRING; 373903.Hore_00600; -.
DR PRIDE; B8D067; -.
DR EnsemblBacteria; ACL68821; ACL68821; Hore_00600.
DR KEGG; hor:Hore_00600; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_9; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000719; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..554
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000185255"
FT BINDING 63..70
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 554 AA; 59069 MW; 2771AA32B81C6321 CRC64;
MKSDIEIAQD ARMEPIEEIA EKAGLHKDQI ELYGKYKAKV NTDIIDDDKK DGKLVLVTAI
TPTPAGEGKT TTTVGLGQAL NLLGKKAIIA LREPSLGPTM GIKGGAAGGG YSQVLPMEDI
NLHFTGDIHA IGAAHNLLSA VIDNHIKQGN DLGIDPTSVT WKRVVDMNDR ALRNIVVGLG
GKAHGVPRED GFMITVASEI MAILCLANNL EDLKNKISNI VVGYTYDGEA ITAGDLKVSG
AMTALLKDAI KPNLVQTLEN TPAFIHGGPF ANIAHGCNSI MATKMALKLG EITVTEAGFG
ADLGAEKFFN IKCRYAGLNP DAAVVVATVR ALKMHGGVDK DSLSEENLDA LARGFENLEK
HLENVSKFGV PSVVAINRFP GDTEAELNLV RDKCEEMGVP VAISEVWARG GEGGLDLARK
LVDVLENTPG QFSYLYDVNF PITDKIEKIA KEIYGADGVN YTSKALKQID KYTELGYDKL
PICMAKTQSS ISDDPSLKGR PRGFRINVRE VNLSAGAGFI IPLTGPVLTM PGLPKKPAAE
GIDIDADGKI SGLF
