FTHS_HALWD
ID FTHS_HALWD Reviewed; 580 AA.
AC Q18JB5;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=HQ_1768A;
OS Haloquadratum walsbyi (strain DSM 16790 / HBSQ001).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloquadratum.
OX NCBI_TaxID=362976;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16790 / HBSQ001;
RX PubMed=16820047; DOI=10.1186/1471-2164-7-169;
RA Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F.,
RA Pfeiffer F., Oesterhelt D.;
RT "The genome of the square archaeon Haloquadratum walsbyi: life at the
RT limits of water activity.";
RL BMC Genomics 7:169-169(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; AM180088; CAJ51896.1; -; Genomic_DNA.
DR RefSeq; WP_011571043.1; NC_008212.1.
DR AlphaFoldDB; Q18JB5; -.
DR SMR; Q18JB5; -.
DR STRING; 362976.HQ_1768A; -.
DR EnsemblBacteria; CAJ51896; CAJ51896; HQ_1768A.
DR GeneID; 4193985; -.
DR KEGG; hwa:HQ_1768A; -.
DR eggNOG; arCOG04541; Archaea.
DR HOGENOM; CLU_003601_3_3_2; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000001975; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..580
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000293074"
FT BINDING 83..90
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 580 AA; 62054 MW; 1BA472E2A03593D6 CRC64;
MTDDSKDTDL SEDNGFPTDY EIAQNADMTP IWDLLEPWDL GNDDLSYYGE YTAKLSHDAV
DHLREEAKTR ENNLILVTGM TPTPMGEGKT VTTVGLGQTL NHLDEEAMIA IREPSLGPVF
GVKGGAAGGG YSQVLPMEEI NLHFTGDLHA LTSAHNLIAA MLDAKLSQSD EFDVDVNDIS
WPRALDMNDR ALRETVIGLG GESGGVPRED GFLLTAASEL MAVLCLSSDL EDLKMRVSRI
IVGYENDGEP ITVDDIDATG PVTMLLRDAI EPNVVQTIEG TPAFVHGGPF ANIAHGTNSL
IADKAAFGMG DYLVTEAGFG SDLGAEKFMN IVCRLGDMTP DAITLVASVR ALKYHGLDMW
PADIDEINGA GVDALEAGFV NLDKHVTNLQ KFGIPVVVAV NRFPNDTDVE VNAVLNHCRN
DLNVKAASST VFAEGSEGGV ELAERVIGAV ESSDPEDFEY LYPADATIKE KIETVATEIY
GAGSVNYSTD AEDDIERMRE LGFDTVPVCL SKTFHSLTDD ARQKGAPTGW TLNVRKLYPS
AGAGFIVALT GDVLTMPGLP ADPAAADMDI DADGDITGLF
