FTHS_HERA2
ID FTHS_HERA2 Reviewed; 570 AA.
AC A9B4H8;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Haur_1499;
OS Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95).
OC Bacteria; Chloroflexi; Chloroflexia; Herpetosiphonales; Herpetosiphonaceae;
OC Herpetosiphon.
OX NCBI_TaxID=316274;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23779 / DSM 785 / 114-95;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C.,
RA Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA Bryant D.A., Richardson P.;
RT "Complete sequence of chromosome of Herpetosiphon aurantiacus ATCC 23779.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000875; ABX04143.1; -; Genomic_DNA.
DR AlphaFoldDB; A9B4H8; -.
DR SMR; A9B4H8; -.
DR STRING; 316274.Haur_1499; -.
DR EnsemblBacteria; ABX04143; ABX04143; Haur_1499.
DR KEGG; hau:Haur_1499; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_0; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000787; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..570
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000146685"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 570 AA; 60778 MW; 98957634815C5115 CRC64;
MKTSLQIAAE ATPRPITQIA EELAIAEQFV EPYGRYRAKI NLDLLDASHD RPRGKQILVT
AMTPTPLGEG KTATTIGLGM ALSRLGKRAI CTLRQSSLGP VFGIKGGGSG GGYSQVIPLE
DSLMHLTGDI HAVTQAHNQI AAMTDNSWYQ KNRLGIDPEQ IQIRRVLDVN DRFLRSITIG
QGGSQHGIPR QTGFDITAAS ELMAILALVS GENHADVMRD LRQRIGRMVV AFTRQGQPIT
ADDIQAAGAA TVIMRNAIHP TLMQTIENTP VLMHGGPFAN IAHGNASVVA DQVGLRIADY
VVTEAGFAMD MGGEKFFDIK CRAFDAKPAV VVLVATIRAL KAHSGRWNIK PGRDLPTDLL
QENPDAVYAG GANLQKHIRN AQLFGLPVVV ALNSFPDDHP SEIEAVREIA MSAGAFDVAV
SKVFSQGGVG GEELAEKVLA AIDQAGQAQF LYELEQPLTA KIATIATKIY GAAEVSYSEA
ASEQLAKLEA NGFGNLPICM AKTHLSISHD PALKGAPTGY SFPIREVRAS IGAGFIYPIA
GDMMTMPGLS ANPAAQQIDI DEHGNTVGLF
