ID   FTHS_LACDA              Reviewed;         559 AA.
AC   Q1GA94;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Ldb1020;
OS   Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081
OS   / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM
OS   00102 / Lb 14).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=390333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB
RC   11778 / NCTC 12712 / WDCM 00102 / Lb 14;
RX   PubMed=16754859; DOI=10.1073/pnas.0603024103;
RA   van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P.,
RA   Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R.,
RA   Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P.,
RA   Weissenbach J., Ehrlich S.D., Maguin E.;
RT   "The complete genome sequence of Lactobacillus bulgaricus reveals extensive
RT   and ongoing reductive evolution.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAI97822.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR954253; CAI97822.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_003619676.1; NZ_JQAV01000013.1.
DR   AlphaFoldDB; Q1GA94; -.
DR   SMR; Q1GA94; -.
DR   STRING; 390333.Ldb1020; -.
DR   PRIDE; Q1GA94; -.
DR   EnsemblBacteria; CAI97822; CAI97822; Ldb1020.
DR   GeneID; 66399933; -.
DR   KEGG; ldb:Ldb1020; -.
DR   PATRIC; fig|390333.13.peg.669; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   BioCyc; LDEL390333:LDB_RS04470-MON; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001259; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..559
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000293039"
FT   BINDING         67..74
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   559 AA;  60503 MW;  750ABAED922A5D41 CRC64;
     MVKSDIEIAQ AAEELPITDV AAKLGLTSQD LEPYGYDKAK VNWQAIKRSE ENGHLGKLIL
     VTSISPTPAG EGKSTMTIGI GDAINNQLGK KTVIALREPS MGPVFGMKGG AAGGGYAQVI
     PMEDINLHFT GDMHALTSAI DNLSALVDNY IYQGNELGLD PEKIVIKRGL DVNDRTLRKV
     TIGQGSKFNG VERPASFQLT VGHELMAILC LSKDIADLKE RIGKVLVGYT YEDEPVFVKD
     LGFQGAIAAL LSTALKPNLV QTLEHTPAFV HGGPFANIAH GNNSILSTNL ALHLSDYVLS
     EAGFGSDLGG QKFLDFVSTK LEKKPDAAVV VATVRALKYQ AEKSTDHLKE ENLDSLKEGF
     ANLDRHMNNV RSYNIPVLVV INKFPTDTEA ELDLLKSLIE EQGFPCEIVT AHDEGSKGAK
     AAAEKIVELA DKSDYEIKRS YDLDDDLETK IEKVAKRIYH AADVEYTDKA KDQLVKLKKM
     GKDKLPVIIA KTQYSFTDNV KELGAPTGFT LHVKGLSLRN GAGFVVVSTG HILDMPGLPK
     HPAALDIDVD ETGKISGLF