FTHS_LEUCK
ID FTHS_LEUCK Reviewed; 554 AA.
AC B1MY45;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=LCK_00614;
OS Leuconostoc citreum (strain KM20).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Leuconostoc.
OX NCBI_TaxID=349519;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KM20;
RX PubMed=18281406; DOI=10.1128/jb.01862-07;
RA Kim J.F., Jeong H., Lee J.-S., Choi S.-H., Ha M., Hur C.-G., Kim J.-S.,
RA Lee S., Park H.-S., Park Y.-H., Oh T.K.;
RT "Complete genome sequence of Leuconostoc citreum KM20.";
RL J. Bacteriol. 190:3093-3094(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; DQ489736; ACA82447.1; -; Genomic_DNA.
DR RefSeq; WP_004903508.1; NC_010471.1.
DR AlphaFoldDB; B1MY45; -.
DR SMR; B1MY45; -.
DR STRING; 349519.LCK_00614; -.
DR EnsemblBacteria; ACA82447; ACA82447; LCK_00614.
DR KEGG; lci:LCK_00614; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_9; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002166; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..554
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000196813"
FT BINDING 64..71
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 554 AA; 58270 MW; 20590D1B6E15559C CRC64;
MQTDIAIAQS AEILPITQIA EKAGLKPNEI LPYGYDKAKI KLDPTISRKK DLGKLILVTS
INPTPAGEGK STVTVGLADA LALAGKKTMI ALREPSLGPV MGMKGGATGG GMSQVIPMAD
INLHFTGDFH ALTAAHDTLA AVVDNSLQQG NPLNIDPRRI IWKRVLDIND RALRHITIGM
GGPTSGVPRE DGFDITVASE LMAILTLSTD LMDLKARVAR IVVGYTYDKV PVTVADLGVS
GALAVLLKDA IMPNLVQTLA HTPAIIHGGP FANIAQGTNS ILATKTALQL ADYTVTEGGF
GADLGGEKFL DVKVPILGKT PDTIVVVATV RALKHHGGVA LADLNNENLT ALAAGLENLG
QHLKAMGRYG VPVVVAINRF SADTDSEIQA IKDYAQAFGA AAYTTTVWAE GGAGAQDLAA
AVIEAADQEA DYTPLYQPEN EAIDKLNAIV TTIYGGLGVE LSATAQKQLM AFKAQGWDKL
PIIMAKTQYS FSDDPKRLGA PKDFVIHVRE FVPKLGAGFL VAMTGSILTM PGLPKHPAAL
DIDIDETGKI TGLF
