FTHS_LISMC
ID FTHS_LISMC Reviewed; 560 AA.
AC C1KWH6;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Lm4b_01893;
OS Listeria monocytogenes serotype 4b (strain CLIP80459).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=568819;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CLIP80459;
RX PubMed=22530965; DOI=10.1186/1471-2164-13-144;
RA Hain T., Ghai R., Billion A., Kuenne C.T., Steinweg C., Izar B.,
RA Mohamed W., Mraheil M., Domann E., Schaffrath S., Karst U., Goesmann A.,
RA Oehm S., Puhler A., Merkl R., Vorwerk S., Glaser P., Garrido P.,
RA Rusniok C., Buchrieser C., Goebel W., Chakraborty T.;
RT "Comparative genomics and transcriptomics of lineages I, II, and III
RT strains of Listeria monocytogenes.";
RL BMC Genomics 13:144-144(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; FM242711; CAS05651.1; -; Genomic_DNA.
DR RefSeq; WP_003728274.1; NC_012488.1.
DR AlphaFoldDB; C1KWH6; -.
DR SMR; C1KWH6; -.
DR KEGG; lmc:Lm4b_01893; -.
DR HOGENOM; CLU_003601_3_3_9; -.
DR OMA; CGEIMTM; -.
DR BioCyc; LMON568819:LM4B_RS09575-MON; -.
DR UniPathway; UPA00193; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..560
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000215438"
FT BINDING 69..76
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 560 AA; 60126 MW; B0D92B76E1DF66BC CRC64;
MSNKVKSDIE IASKAEILPV TTIAEHLGLD ADALELYGKY KAKLSYDTIH SLKDKEPGKL
VLVTAINPTP AGEGKSTVTV GLGDALSKKD KKTVIALREP SLGPTMGIKG GATGGGYAQV
IPMEDINLHF TGDFHAITAA NNALSAFIDN HMQQGNDLDI DGRRIVWKRV VDLNDRALRK
VVVGLGGPIQ GVPREDGFDI TVASEIMAII CLASDLKDLK KRLSEIVIGY NYKKEPITVG
EMGYEGALTL LLKDALKPNL VQTLEHTPAI VHGGPFANIA HGCNSVSATS TALRLGEYVV
TEAGFGADLG AEKFLDIKVP ALGKAPDCVV IVATIRALKM HGGALKTELS EENVDALAKG
FTNLQKHTES IQTFGIPYVV AINKFITDSD AEVAKLEALC EEHGIPFSLT EVWEKGGDGG
LELADKVIAA VESGEADYKR IYDDAWSIEE KLEAIVTKVY GGIGVELSSK AQKQIVEFKK
YGWDRYPICM AKTQYSLSDD PTLLGRPTDF VIHIREFIPK LGAGFVVALT GDVMTMPGLP
KKPAALNMDV DENGNAQGLF
