ID   FTHS_LISMH              Reviewed;         560 AA.
AC   B8DDM6;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=LMHCC_0680;
OS   Listeria monocytogenes serotype 4a (strain HCC23).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=552536;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HCC23;
RX   PubMed=21602330; DOI=10.1128/jb.05236-11;
RA   Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T., Bridges S.M.,
RA   Lawrence M.L.;
RT   "Genome sequence of lineage III Listeria monocytogenes strain HCC23.";
RL   J. Bacteriol. 193:3679-3680(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP001175; ACK39035.1; -; Genomic_DNA.
DR   RefSeq; WP_012581091.1; NC_011660.1.
DR   AlphaFoldDB; B8DDM6; -.
DR   SMR; B8DDM6; -.
DR   KEGG; lmh:LMHCC_0680; -.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   OMA; CGEIMTM; -.
DR   UniPathway; UPA00193; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..560
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000185256"
FT   BINDING         69..76
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   560 AA;  60130 MW;  ED5D2B76E1DF66BD CRC64;
     MSNKVKSDIE IASKAEILPV TTIAEHLGLD ADALELYGKY KAKLSYDTIH SLKDKETGKL
     VLVTAINPTP AGEGKSTVTV GLGDALSKKD KKTVIALREP SLGPTMGIKG GATGGGYAQV
     IPMEDINLHF TGDFHAITAA NNALSAFIDN HMQQGNDLDI DGRRIVWKRV VDLNDRALRK
     VVVGLGGPIQ GVPREDGFDI TVASEIMAII CLASDLKDLK KRLSEIVIGY NYKKEPITVG
     EMGYEGALTL LLKDALKPNL VQTLEHTPAI VHGGPFANIA HGCNSVSATS TALRLGEYVV
     TEAGFGADLG AEKFLDIKVP ALGKAPDCVV IVATIRALKM HGGALKTELS EENVDALAKG
     FTNLQKHTES IQTFGIPYVV AINKFITDSD AEVAKLEALC EEHGIPFSLT EVWEKGGDGG
     LELADKVIAA VESGEADYKR IYDDAWSIEE KLEAIVTKVY GGIGVELSSK AQKQIVEFKK
     YGWDRYPICM AKTQYSLSDD PTLLGRPTDF VIHIREFIPK LGAGFVVALT GDVMTMPGLP
     KKPAALNMDV DENGNAQGLF