ID   FTHS_LISMO              Reviewed;         560 AA.
AC   Q8Y624;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=lmo1877;
OS   Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=169963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-679 / EGD-e;
RX   PubMed=11679669; DOI=10.1126/science.1063447;
RA   Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA   Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA   Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA   Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA   Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA   Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA   Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA   Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA   Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA   Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT   "Comparative genomics of Listeria species.";
RL   Science 294:849-852(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC99955.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AL591981; CAC99955.1; ALT_FRAME; Genomic_DNA.
DR   PIR; AE1309; AE1309.
DR   RefSeq; NP_465401.1; NC_003210.1.
DR   AlphaFoldDB; Q8Y624; -.
DR   SMR; Q8Y624; -.
DR   STRING; 169963.lmo1877; -.
DR   PaxDb; Q8Y624; -.
DR   EnsemblBacteria; CAC99955; CAC99955; CAC99955.
DR   GeneID; 985815; -.
DR   KEGG; lmo:lmo1877; -.
DR   PATRIC; fig|169963.11.peg.1922; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   PhylomeDB; Q8Y624; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000817; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..560
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199357"
FT   BINDING         69..76
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   560 AA;  60086 MW;  598BC995BC7A0AA3 CRC64;
     MSNKVKSDIE IASKAEILPV TTIAEHLGLD ADALELYGKY KAKLSYDTIH SLKDKEPGKL
     VLVTAINPTP AGEGKSTVTV GLGDALSKKD KKTVIALREP SLGPTMGIKG GATGGGYAQV
     IPMEDINLHF TGDFHAITAA NNALSAFIDN HMQQGNDLEI DGRRIVWKRV VDLNDRALRK
     VVVGLGGPIQ GVPREDGFDI TVASEIMAII CLASDLKDLK KRLSEIVIGY NYKKEPITVG
     EMGYEGALTL LLKDALKPNL VQTLEHTPAI VHGGPFANIA HGCNSVSATS TALRLGDYVV
     TEAGFGADLG AEKFLDIKVP ALGKAPDCVV IVATIRALKM HGGALKTELS EENVDALAKG
     FTNLQKHTES IQTFGIPYVV AINKFITDSD AEVAKLEALC EEHGIPFSLT EVWEKGGDGG
     LELADKVIAA VESGAADYKR IYDDAWSMEE KLEAIVTKVY GGIGVELSSK AQKQIVEFKK
     YGWDRYPICM AKTQYSLSDD PTLLGRPTDF VIHIREFIPK LGAGFVVALT GDVMTMPGLP
     KKPAALNMDV DENGNAQGLF