FTHS_METC4
ID FTHS_METC4 Reviewed; 557 AA.
AC B7L0A5;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Mchl_0447;
OS Methylorubrum extorquens (strain CM4 / NCIMB 13688) (Methylobacterium
OS extorquens).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylorubrum.
OX NCBI_TaxID=440085;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CM4 / NCIMB 13688;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA Marx C., Richardson P.;
RT "Complete sequence of chromosome of Methylobacterium chloromethanicum
RT CM4.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP001298; ACK81383.1; -; Genomic_DNA.
DR RefSeq; WP_012605559.1; NC_011757.1.
DR PDB; 7C11; X-ray; 2.81 A; A/B/C/D/M/N/O/P=1-557.
DR PDBsum; 7C11; -.
DR AlphaFoldDB; B7L0A5; -.
DR SMR; B7L0A5; -.
DR PRIDE; B7L0A5; -.
DR EnsemblBacteria; ACK81383; ACK81383; Mchl_0447.
DR KEGG; mch:Mchl_0447; -.
DR HOGENOM; CLU_003601_3_3_5; -.
DR OMA; CGEIMTM; -.
DR BioCyc; MEXT440085:MCHL_RS02080-MON; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002385; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Ligase; Nucleotide-binding;
KW One-carbon metabolism.
FT CHAIN 1..557
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000185257"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
FT HELIX 4..10
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 16..21
FT /evidence="ECO:0007829|PDB:7C11"
FT TURN 22..24
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 27..29
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 30..33
FT /evidence="ECO:0007829|PDB:7C11"
FT TURN 34..36
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 37..40
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 42..46
FT /evidence="ECO:0007829|PDB:7C11"
FT TURN 47..50
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 55..63
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 71..84
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 89..93
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 98..102
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 109..111
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 114..117
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 119..123
FT /evidence="ECO:0007829|PDB:7C11"
FT TURN 124..127
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 129..150
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 156..161
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 165..169
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 172..174
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 175..180
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 184..186
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 190..192
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 194..196
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 197..199
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 201..206
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 212..220
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 223..227
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 232..234
FT /evidence="ECO:0007829|PDB:7C11"
FT TURN 235..239
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 241..248
FT /evidence="ECO:0007829|PDB:7C11"
FT TURN 249..252
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 255..259
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 264..267
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 273..276
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 282..291
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 293..298
FT /evidence="ECO:0007829|PDB:7C11"
FT TURN 303..305
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 306..312
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 314..318
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 323..329
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 331..337
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 350..369
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 374..379
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 386..400
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 404..407
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 409..412
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 413..417
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 418..429
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 441..443
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 445..456
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 459..464
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 466..477
FT /evidence="ECO:0007829|PDB:7C11"
FT TURN 478..480
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 481..483
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 485..488
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 491..497
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 506..511
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 513..517
FT /evidence="ECO:0007829|PDB:7C11"
FT TURN 518..521
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 522..526
FT /evidence="ECO:0007829|PDB:7C11"
FT HELIX 541..544
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 549..551
FT /evidence="ECO:0007829|PDB:7C11"
FT STRAND 553..555
FT /evidence="ECO:0007829|PDB:7C11"
SQ SEQUENCE 557 AA; 59478 MW; 969EF0DC2DE42C8B CRC64;
MPSDIEIARA ATLKPIAQVA EKLGIPDEAL HNYGKHIAKI DHDFIASLEG KPEGKLVLVT
AISPTPAGEG KTTTTVGLGD ALNRIGKRAV MCLREPSLGP CFGMKGGAAG GGKAQVVPME
QINLHFTGDF HAITSAHSLA AALIDNHIYW ANELNIDVRR IHWRRVVDMN DRALRAINQS
LGGVANGFPR EDGFDITVAS EVMAVFCLAK NLADLEERLG RIVIAETRDR KPVTLADVKA
TGAMTVLLKD ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRTGLR LADYTVTEAG
FGADLGAEKF IDIKCRQTGL KPSSVVIVAT IRALKMHGGV NKKDLQAENL DALEKGFANL
ERHVNNVRSF GLPVVVGVNH FFQDTDAEHA RLKELCRDRL QVEAITCKHW AEGGAGAEAL
AQAVVKLAEG EQKPLTFAYE TETKITDKIK AIATKLYGAA DIQIESKAAT KLAGFEKDGY
GKLPVCMAKT QYSFSTDPTL MGAPSGHLVS VRDVRLSAGA GFVVVICGEI MTMPGLPKVP
AADTIRLDAN GQIDGLF
