FTHS_METFK
ID FTHS_METFK Reviewed; 557 AA.
AC Q1GZ16;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Mfla_2254;
OS Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875).
OC Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC Methylophilaceae; Methylobacillus.
OX NCBI_TaxID=265072;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KT / ATCC 51484 / DSM 6875;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J.,
RA Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.;
RT "Complete sequence of Methylobacillus flagellatus KT.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000284; ABE50521.1; -; Genomic_DNA.
DR RefSeq; WP_011480475.1; NC_007947.1.
DR AlphaFoldDB; Q1GZ16; -.
DR SMR; Q1GZ16; -.
DR STRING; 265072.Mfla_2254; -.
DR PRIDE; Q1GZ16; -.
DR EnsemblBacteria; ABE50521; ABE50521; Mfla_2254.
DR KEGG; mfa:Mfla_2254; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_4; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002440; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..557
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000293047"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 557 AA; 60157 MW; 8C3936EB1B4889D0 CRC64;
MFSDIEIARQ AKLRKISEIA AELGLNEDSI TLYGQYKAKI DLNANPQLQA QPDGKLILVT
AISPSPAGEG KTTTTVGLGD ALQRMGKRAV ICLREPSMGP VFGMKGGAAG GGYAQVVPME
DINLHFTGDF ASIALAHNLL AAMIDNHIHH GNALQIDPRQ VTWKRVIDMN DRALRQVVIG
LGGKVNGQPR EDGFDIVVAS EVMAVFCLAD SLKDLRTRLG RIVVGYTYSG QPVTAQDLKA
HGAMTVLLKD AIKPNLVQTL EHTPALLHGG PFANIAHGCN SVIATRTALK LGDYVVTEAG
FGADLGAEKF IDIKCRKAGL HPDACVIVAT IRALKYHGGA DVSALSREDM LALDRGIANL
EKHVENVTTH YGMPAVIALN RFDHDTEAEL ALVMKRMGEM GIKVVVAEHW AKGGAGAESL
AAHVLQLIEG RSEGVRYVYE SSETLWEKVN QVARKIYGAM DVHAPRPVRA MIDRLQDAGY
GHYPICIAKT QYSFSTDASR RGVPHEHILT VRAVRLCAGA EFVVIICDDI MTMPGLPEEP
CAIRMDIDDD GQVVGLY