FTHS_METLZ
ID FTHS_METLZ Reviewed; 560 AA.
AC A2SS72;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Mlab_1009;
OS Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
OX NCBI_TaxID=410358;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43576 / DSM 4855 / Z;
RX PubMed=21304657; DOI=10.4056/sigs.35575;
RA Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A.,
RA Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L.,
RA Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.;
RT "Complete genome sequence of Methanocorpusculum labreanum type strain Z.";
RL Stand. Genomic Sci. 1:197-203(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000559; ABN07178.1; -; Genomic_DNA.
DR AlphaFoldDB; A2SS72; -.
DR SMR; A2SS72; -.
DR STRING; 410358.Mlab_1009; -.
DR EnsemblBacteria; ABN07178; ABN07178; Mlab_1009.
DR KEGG; mla:Mlab_1009; -.
DR eggNOG; arCOG04541; Archaea.
DR HOGENOM; CLU_003601_3_3_2; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000365; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..560
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000293075"
FT BINDING 70..77
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 560 AA; 59858 MW; C8466B9193943ECA CRC64;
MGWKYLLSDI EIAQQCKMKK ITEIAASLDI TPDELELYGS YKAKLADSLE KRLADKPNGK
LILVTAINPT PAGEGKTTTT VGLGQAMPKI GKKAVIALRE PSLGPVFGVK GGAAGGGYSQ
VVPMEDINLH FTGDFHAITS ANNLLCAMID NHIQQGNALD IDTRRIIFKR CLDMNDRALR
NIIIGLGGQT NGVPREDHFM ITVASEVMAI LCLANDIDDL KERLGNVIFG YSRKGTPLYA
RDLKAVGAMA ALLKDAIKPN LVQTLENTPC FIHGGPFANI AHGCNSVRAT KLSLKMADYV
ITEAGFGSDL GAEKFFDIKC RYAGLTPNTV VLVATVRALK YNGGVKKEDT TIPNVAALKA
GMVNLEAHIQ NLQTFGVPVV VAINRFSTDT DEELAVLKEF CTAQGAEFAI SEVFAKGGEG
GVELAKKVVA SCEKPQKFQC LYELNTPIKE KINALATRIY GADGVVYSPA ADAAIKDIDA
LGRSNLPICM AKTQYSLSDD PNKLGRPKNF VINAATVRLC NGAGFIVVET GDIMTLPGLP
AVPAACSIDV NNDGYISGLF
