FTHS_MOOTA
ID FTHS_MOOTA Reviewed; 559 AA.
AC Q2RM91;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Moth_0109;
OS Moorella thermoacetica (strain ATCC 39073 / JCM 9320).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Moorella group; Moorella.
OX NCBI_TaxID=264732;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39073 / JCM 9320;
RX PubMed=18631365; DOI=10.1111/j.1462-2920.2008.01679.x;
RA Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C.,
RA Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W.;
RT "The complete genome sequence of Moorella thermoacetica (f. Clostridium
RT thermoaceticum).";
RL Environ. Microbiol. 10:2550-2573(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000232; ABC18448.1; -; Genomic_DNA.
DR RefSeq; WP_011391657.1; NC_007644.1.
DR RefSeq; YP_428991.1; NC_007644.1.
DR PDB; 4IOJ; X-ray; 2.20 A; A/B=1-559.
DR PDB; 4IOK; X-ray; 2.50 A; A/B=1-559.
DR PDB; 4IOL; X-ray; 2.56 A; A/B=1-559.
DR PDB; 4IOM; X-ray; 3.00 A; A/B=1-559.
DR PDBsum; 4IOJ; -.
DR PDBsum; 4IOK; -.
DR PDBsum; 4IOL; -.
DR PDBsum; 4IOM; -.
DR AlphaFoldDB; Q2RM91; -.
DR SMR; Q2RM91; -.
DR STRING; 264732.Moth_0109; -.
DR PRIDE; Q2RM91; -.
DR EnsemblBacteria; ABC18448; ABC18448; Moth_0109.
DR KEGG; mta:Moth_0109; -.
DR PATRIC; fig|264732.11.peg.114; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_9; -.
DR OMA; TRQGFSK; -.
DR UniPathway; UPA00193; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Ligase; Nucleotide-binding;
KW One-carbon metabolism.
FT CHAIN 1..559
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000300530"
FT BINDING 68..75
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
FT HELIX 7..12
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 19..25
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 30..32
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 33..39
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 41..43
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 45..50
FT /evidence="ECO:0007829|PDB:4IOJ"
FT TURN 51..53
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 58..65
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 74..87
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 92..96
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 102..105
FT /evidence="ECO:0007829|PDB:4IOJ"
FT TURN 106..108
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 117..120
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 122..126
FT /evidence="ECO:0007829|PDB:4IOJ"
FT TURN 127..130
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 132..152
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 161..163
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 168..172
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 175..177
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 178..182
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 187..189
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 193..195
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 197..199
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 200..202
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 204..211
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 215..224
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 226..230
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 235..237
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 239..241
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 244..250
FT /evidence="ECO:0007829|PDB:4IOJ"
FT TURN 251..255
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 258..262
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 267..270
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 276..279
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 285..294
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 296..305
FT /evidence="ECO:0007829|PDB:4IOJ"
FT TURN 306..308
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 309..315
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 317..321
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 327..332
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 334..340
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 345..347
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 353..371
FT /evidence="ECO:0007829|PDB:4IOJ"
FT TURN 372..374
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 377..382
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 389..401
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 405..409
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 411..414
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 415..419
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 420..432
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 447..457
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 464..466
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 468..479
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 496..498
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 511..513
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 515..519
FT /evidence="ECO:0007829|PDB:4IOJ"
FT TURN 520..523
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 524..528
FT /evidence="ECO:0007829|PDB:4IOJ"
FT HELIX 543..546
FT /evidence="ECO:0007829|PDB:4IOJ"
FT STRAND 555..557
FT /evidence="ECO:0007829|PDB:4IOJ"
SQ SEQUENCE 559 AA; 59942 MW; D8CD2BB52FC35A4D CRC64;
MSKVPSDIEI AQAAKMKPVM ELARGLGIQE DEVELYGKYK AKISLDVYRR LKDKPDGKLI
LVTAITPTPA GEGKTTTSVG LTDALARLGK RVMVCLREPS LGPSFGIKGG AAGGGYAQVV
PMEDINLHFT GDIHAVTYAH NLLAAMVDNH LQQGNVLNID PRTITWRRVI DLNDRALRNI
VIGLGGKANG VPRETGFDIS VASEVMACLC LASDLMDLKE RFSRIVVGYT YDGKPVTAGD
LEAQGSMALL MKDAIKPNLV QTLENTPAFI HGGPFANIAH GCNSIIATKT ALKLADYVVT
EAGFGADLGA EKFYDVKCRY AGFKPDATVI VATVRALKMH GGVPKSDLAT ENLEALREGF
ANLEKHIENI GKFGVPAVVA INAFPTDTEA ELNLLYELCA KAGAEVALSE VWAKGGEGGL
ELARKVLQTL ESRPSNFHVL YNLDLSIKDK IAKIATEIYG ADGVNYTAEA DKAIQRYESL
GYGNLPVVMA KTQYSFSDDM TKLGRPRNFT ITVREVRLSA GAGFIVPITG AIMTMPGLPK
RPAACNIDID ADGVITGLF
