ID   FTHS_NOCSJ              Reviewed;         559 AA.
AC   A1SQH3;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Noca_4561;
OS   Nocardioides sp. (strain ATCC BAA-499 / JS614).
OC   Bacteria; Actinobacteria; Propionibacteriales; Nocardioidaceae;
OC   Nocardioides.
OX   NCBI_TaxID=196162;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-499 / JS614;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J.,
RA   Richardson P.;
RT   "Complete sequence of chromosome 1 of Nocardioides sp. JS614.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000509; ABL84058.1; -; Genomic_DNA.
DR   RefSeq; WP_011757986.1; NC_008699.1.
DR   AlphaFoldDB; A1SQH3; -.
DR   SMR; A1SQH3; -.
DR   STRING; 196162.Noca_4561; -.
DR   EnsemblBacteria; ABL84058; ABL84058; Noca_4561.
DR   KEGG; nca:Noca_4561; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_11; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000640; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..559
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000293049"
FT   BINDING         66..73
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   559 AA;  58921 MW;  6CCE6AECED3646C3 CRC64;
     MLSDIEIAGA ATLRPITEVA TESLGIGAEH LVPYGHYKAK VGITYLNSLA DRPLGRLILV
     TALSPTPPGE GKTTTSVGLT DALHGLGKRA IACLREPSMG PVFGLKGGAA GGGYSQVVPM
     TDINLHFTGD FAAIAAANNL LAALIDNHVH HGNELDIDVR SVTWKRVLDT NDRALREVVV
     GLGGPPNGFP RQDGFDIVVA SELMAIFCLT ESWADLKRRI GDIVIGYSRA GAPVTARDLG
     ADGAMAVLLR DAIAPNLVQT LEGAPALVHG GPFANIAHGC SSVMATRAGL RLADYVVTEA
     GFGADLGAEK FIDIKCRMSG MRPDVAVVVA TVRALKYHGG VALADLDRED LGAVEAGMDN
     LRRHLDNLRH LNGVPCVVAV NRFPTDTDLE VVRVVELAAS YGVPAYQATH FTDGGIGAQD
     LAKGVLQALE EPARDEFSFT YPDELSLTEK VEAVATRVYG AGQVTWDGKA RKRLARIERD
     GYGTLPVCVA KTQYSFSTDP GLLGAPTGHE LRVREVRLSA GAGFVVVICG DMMTMPGLPT
     RPAATRIDLA DDGTIIGLS