FTHS_PAEAT
ID FTHS_PAEAT Reviewed; 562 AA.
AC A1R8N9;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=AAur_2892;
OS Paenarthrobacter aurescens (strain TC1).
OC Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Paenarthrobacter.
OX NCBI_TaxID=290340;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TC1;
RX PubMed=17194220; DOI=10.1371/journal.pgen.0020214;
RA Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B.,
RA Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V.,
RA Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J.;
RT "Secrets of soil survival revealed by the genome sequence of Arthrobacter
RT aurescens TC1.";
RL PLoS Genet. 2:2094-2106(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000474; ABM10024.1; -; Genomic_DNA.
DR RefSeq; WP_011775541.1; NC_008711.1.
DR AlphaFoldDB; A1R8N9; -.
DR SMR; A1R8N9; -.
DR STRING; 290340.AAur_2892; -.
DR EnsemblBacteria; ABM10024; ABM10024; AAur_2892.
DR KEGG; aau:AAur_2892; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_11; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000637; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..562
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000293031"
FT BINDING 70..77
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 562 AA; 59201 MW; 994851454A18EF7C CRC64;
MSENKVLSDL EIAHNATMLP VEAIAERAGI NVDALELYGP YKAKINTAKL VLPRGKAPGK
VVLVSAMSPT PAGEGKSTTT VGLADSLARA GHKVMIALRE PSLGPILGMK GGATGGGYSQ
VLPMDDINLH FTGDFHAITS ANNALMALVD NHIFQGNQLG IDPRRMTFKR VLDMNDRALR
EVVIGLGGPM QGVPRQDGFD ITVASEIMAV FCLATDLDDL RERLGRITFG YTYDRTPVTV
SDLGVEGALT MLLKDAIKPN LVQTIAGTPA LVHGGPFANI AHGCNSLIAT STAMQLADIV
VTEAGFGADL GAEKYMDIKA RIAEVAPSAV VVVATIRALK MQGGVPKEKL NEPNVEAVAA
GVENLKRHVG NVAKFGISPV VSINKFGSDT PEELEWLLAW CAAEGVEAAV ADVWGRGGGG
DGGDELAAKV AAALEAPSNF HHLYPLELGV EDKIRTIVQE IYGADGVEFS VPALKRLAEI
EKNGWSGLPV CMAKTQYSFT DDASRLGAPK GFRVHVRELI PKTGAGFIVA LTGAVMTMPG
LPKEPAAMRM DVDADGNPTG LF
