FTHS_PELUB
ID FTHS_PELUB Reviewed; 561 AA.
AC Q4FL49;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=SAR11_1285;
OS Pelagibacter ubique (strain HTCC1062).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales;
OC Pelagibacteraceae; Candidatus Pelagibacter.
OX NCBI_TaxID=335992;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTCC1062;
RX PubMed=16109880; DOI=10.1126/science.1114057;
RA Giovannoni S.J., Tripp H.J., Givan S., Podar M., Vergin K.L., Baptista D.,
RA Bibbs L., Eads J., Richardson T.H., Noordewier M., Rappe M.S., Short J.M.,
RA Carrington J.C., Mathur E.J.;
RT "Genome streamlining in a cosmopolitan oceanic bacterium.";
RL Science 309:1242-1245(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000084; AAZ22089.1; -; Genomic_DNA.
DR RefSeq; WP_011282279.1; NC_007205.1.
DR AlphaFoldDB; Q4FL49; -.
DR SMR; Q4FL49; -.
DR STRING; 335992.SAR11_1285; -.
DR PRIDE; Q4FL49; -.
DR EnsemblBacteria; AAZ22089; AAZ22089; SAR11_1285.
DR GeneID; 66295776; -.
DR KEGG; pub:SAR11_1285; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_5; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002528; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..561
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199366"
FT BINDING 70..77
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 561 AA; 61012 MW; 001C108301484770 CRC64;
MSEVKSDIQI AREAKMQPIN DILAKINVPD ESSAFSPMGR HIAKINLEYL DTLKDKPNGK
LVLVTAITPT PAGEGKTTTS VGLNDGLNKI GKKSIVCLRE PSLGPSFGMK GGAAGGGYAQ
VVPMEQINLH FTGDFHAITS AHNLLSALID NHIYWGNKLD IDVRRIVWKR VMDMNDRSLR
SININLGGVA NGFPREDGFD ITVASEIMAI FCLSNDLEDL EKRIGNITIA YTRDKKPVYA
KDLKAQGPMT VLLKDAIRPN VTQTLENNPA IIHGGPFANI AHGCNSVIAT KTGLKLADYV
VTEAGFGADL GAEKFLDIKC RKSDLKPSCV VIVATIRALK MHGGVAKDDL KTENVEALKK
GLVNLQRHVE NVKKFGLPVA VAVNHFIKDT ENEVKALIEF CDTIGVKASL CTHWANGGEG
TKELASHVAE LCEKNEDKFK FLYESKTPLF KKIETIAKEI YRADEVIADT KIRDQLKSFE
DAGYGDFPIC IAKTQYSFST DPSLKGAPTG HSLPIREIKL SSGAEFIVVI CGAVMTMPGL
PRVPAADSIK LNKDGEIEGL F
