ID   FTHS_PEPAC              Reviewed;         556 AA.
AC   Q5XZD9;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; Synonyms=thf;
OS   Peptoclostridium acidaminophilum (Eubacterium acidaminophilum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Peptoclostridium.
OX   NCBI_TaxID=1731;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Poehlein A., Lechel A., Groebe D., Andreesen J.R.;
RT   "Genes involved in glycine decarboxylation reaction of Eubacterium
RT   acidaminophilum.";
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY722711; AAU84895.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5XZD9; -.
DR   SMR; Q5XZD9; -.
DR   UniPathway; UPA00193; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..556
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199348"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   556 AA;  59161 MW;  C76CAAAA272D0986 CRC64;
     MKTDVQIAQE AKMLPIMEVA KQIGLGEDDI ELYGKYKAKI SLDVYKRLAD KPDGKLVLVT
     AINPTPAGEG KTTTNVGLSM GLNKIGKKTI TALNEPSLGP CFGVKGGAAG GGYAQVVPMD
     DINLHFTGDI HAITTAHNLL AALMDNHIKQ GNALGIDINK ITWKRVLDMN DRALRDIVIG
     LGGTANGIPR QDGFDITVAS EIMAIMCLAT SLSDLKDRLS RMIVGYTSRR LAVTADSLTL
     RGALALLLKD ALKPNLVQTL ENTPAIIHGG PFANIAHGCN SVTTTKTALK IADYVVTEAG
     FGADLGAEKF FDIKCRFADL KPDVAVIVAT VRALKNHGGV AKANLGAENM KALEDGFGNL
     ERHIENVHKF GVPAVVAINA FPTDTEKELK FVEDACRKLG ADVVLSEVWA KGGEGGVELA
     KKVVEVTEKG AAKFKPLYPA EMPLKQKIET IAKEIYRADG VEFSAKASKE LDKFEKLGFG
     NLPICVAKTQ YSFSDNPNLK GAPKGFTVSV SNARISAGAG FIVVLTGDIM TMPGLPKVPA
     ANHMDVLESG EIVGLF