FTHS_PHOV8
ID FTHS_PHOV8 Reviewed; 555 AA.
AC A6L4P0;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=BVU_3016;
OS Phocaeicola vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / CCUG 4940 /
OS NBRC 14291 / NCTC 11154) (Bacteroides vulgatus).
OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Phocaeicola.
OX NCBI_TaxID=435590;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8482 / DSM 1447 / JCM 5826 / CCUG 4940 / NBRC 14291 / NCTC
RC 11154;
RX PubMed=17579514; DOI=10.1371/journal.pbio.0050156;
RA Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C.,
RA Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H.,
RA Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K.,
RA Knight R.D., Gordon J.I.;
RT "Evolution of symbiotic bacteria in the distal human intestine.";
RL PLoS Biol. 5:1574-1586(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000139; ABR40654.1; -; Genomic_DNA.
DR RefSeq; WP_005846873.1; NC_009614.1.
DR AlphaFoldDB; A6L4P0; -.
DR SMR; A6L4P0; -.
DR STRING; 435590.BVU_3016; -.
DR EnsemblBacteria; ABR40654; ABR40654; BVU_3016.
DR KEGG; bvu:BVU_3016; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_10; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR BioCyc; BVUL435590:G1G59-3138-MON; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002861; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..555
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000300516"
FT BINDING 64..71
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 555 AA; 60524 MW; 771F49D9C92B5CE4 CRC64;
MKTDIEIARS IELVKIKQVA RETGIPVEHI SNYGRYIAKV DESQINEEKV QQSNLILVTA
ITPTKAGIGK TTVSIGLALG LNKIGKKTIV ALREPSLGPC FGMKGGAAGG GYAQVLPMDK
INLHFTGDFH AITSAHNMIS ALLDNYLYQN RDNGFGLKEV LWRRVLDVND RSLRYIVTGL
GPKTNGITQE SGFDITPASE IMAILCLAKD EKDLRRRIEN ILLGFTYDNK PFTVKDLGVA
GAITVLLKDA LSPNLVQTTE HTAAFVHGGP FANIAHGCNS ILATKMAMTF SDYTITEAGF
GADLGAEKFY DIKCRKAGVT PKLTVLVVTA RALKMHGGVS QDKIKEPNLE ALKQGVANMD
KHLRNLRYFG QTVVVAFNRY GDDSEEEVDY IRTHCEKKGV GFAVNNAFTD GGEGAVELAE
LVVKTIEEQP SEPLQYAYDD EDSVETKISK VACNLYGASI ITYSAAARKK LKHIVELGYG
NFPICIAKTQ YSFSTDPKIY GAVDNFEFHV QDIVMNAGAE MLVVIAGEIM RMPGLPKEPQ
ALHIDIVNGE IEGLS