ID   FTHS_PICTO              Reviewed;         535 AA.
AC   Q6KZM3;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=PTO1244;
OS   Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC
OS   100828).
OC   Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC   Picrophilaceae; Picrophilus.
OX   NCBI_TaxID=263820;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828;
RX   PubMed=15184674; DOI=10.1073/pnas.0401356101;
RA   Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C.,
RA   Schepers B., Dock C., Antranikian G., Liebl W.;
RT   "Genome sequence of Picrophilus torridus and its implications for life
RT   around pH 0.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AE017261; AAT43829.1; -; Genomic_DNA.
DR   RefSeq; WP_011178045.1; NC_005877.1.
DR   AlphaFoldDB; Q6KZM3; -.
DR   SMR; Q6KZM3; -.
DR   STRING; 263820.PTO1244; -.
DR   PRIDE; Q6KZM3; -.
DR   EnsemblBacteria; AAT43829; AAT43829; PTO1244.
DR   GeneID; 2844250; -.
DR   KEGG; pto:PTO1244; -.
DR   PATRIC; fig|263820.9.peg.1292; -.
DR   eggNOG; arCOG04541; Archaea.
DR   HOGENOM; CLU_003601_3_3_2; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 15172at2157; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000438; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..535
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199414"
FT   BINDING         50..57
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   535 AA;  58003 MW;  73212E2A14961B67 CRC64;
     MKNISEIINI PEDYYDLYGR YIAKVSLNVL DYLKNKRYGK LILVTAMTPT PAGEGKTTTA
     IGLGNALKLL GKNAGIAIRE PSLGPCFGVK GGATGGGKST VEPSNKINLM FTGDFPAVSA
     AHNLLSAVIN NHMYHGNELK LDPKNIVFPR TIDMDDRSLR SIIVGSGDRS TGVMMNDKYV
     ITAASEVMAI LALSRNYNEL KQRLGNIMIG YNLNKAPIFA RDLKVHGAMA SLLVDALRPN
     IAQTSEHVPA IIHTGPFGNI AHGTSSILGD IIGLKMFDYL VTEAGFGSDL GFEKFIDIVL
     RLSDFKLSAV VLVATVRAMR YHGGGRINEP DVNAVLRGSE NLMWHVQNIK KFGFNPVVAI
     NRHSNDTDAE INAISNILTK NGVEFSISDA YSDGGHGALD LAGKVLKSIS DYNPRYIYGI
     NDDPEEKISK IAMNVYNANS VEFSHDAVKT MKLIKDDFSD LYVCMAKTQS SISDNAKLIN
     VPEGFTVKIN GININSGSGF IIPLLGNIMT MPGLPRRPAS ENIDIDDYGN ITGLQ