FTHS_RHIE6
ID FTHS_RHIE6 Reviewed; 559 AA.
AC B3PV59;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN OrderedLocusNames=RHECIAT_CH0003236;
OS Rhizobium etli (strain CIAT 652).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=491916;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CIAT 652;
RA Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P.,
RA Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J.,
RA Palacios R., Davila G.;
RT "Genome diversity and DNA divergence of Rhizobium etli.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP001074; ACE92184.1; -; Genomic_DNA.
DR RefSeq; WP_012484697.1; NC_010994.1.
DR AlphaFoldDB; B3PV59; -.
DR SMR; B3PV59; -.
DR EnsemblBacteria; ACE92184; ACE92184; RHECIAT_CH0003236.
DR GeneID; 45958492; -.
DR KEGG; rec:RHECIAT_CH0003236; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_5; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000008817; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..559
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000196821"
FT BINDING 68..75
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 559 AA; 59746 MW; 19AE39244852769A CRC64;
MPSIKSDIEI ARAAAKKPIF EIGAQLGIPV EQLAPYGHDK AKVSAEFIAA QAGRKDGKLI
LVTAINPTPA GEGKTTTTVG LGDGLNRIGK KAIICIREAS LGPCFGVKGG AAGGGYAQVV
PMEDINLHFT GDFHAITSAH NLLAAMIDNH IYWGNEENID IRRIAWRRVM DMNDRALRSM
VSSLGGVANG FPRQGGFDIT VASEVMAILC LATDLKDLER RLGDIIIGYR FDKTPVHARD
LKADGAMAVL LKDAMQPNLV QTLENNPAFV HGGPFANIAH GCNSVTATKT ALKLGDYVVT
EAGFGADLGA EKFFDIKCRK AGLRPDAAVI VATVRALKMN GGVKKEDLGT EDVAALKKGC
ANLGRHVANV RRFGVPVVVA INHFVSDTDA EIAAVKEFVS RLGAEAILCQ HWAKGSAGIE
ELAHKVVELA ESGQAKFHPL YGDDLSLFEK IEIIASKIYH AGEVTADKAV RDQLQSWEEQ
GYGKLPVCMA KTQYSFSTDP NLRGAPEGHI VSVREVRLSA GAGFVVAITG EIMTMPGLPK
SPSAERIFLN DQGYIEGLF
