FTHS_RUEPO
ID FTHS_RUEPO Reviewed; 558 AA.
AC Q5LNV0;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs1 {ECO:0000255|HAMAP-Rule:MF_01543}; Synonyms=fhs-1;
GN OrderedLocusNames=SPO1557;
GN and
GN Name=fhs2 {ECO:0000255|HAMAP-Rule:MF_01543}; Synonyms=fhs-2;
GN OrderedLocusNames=SPO3103;
OS Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter
OS pomeroyi).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Ruegeria.
OX NCBI_TaxID=246200;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700808 / DSM 15171 / DSS-3;
RX PubMed=15602564; DOI=10.1038/nature03170;
RA Moran M.A., Buchan A., Gonzalez J.M., Heidelberg J.F., Whitman W.B.,
RA Kiene R.P., Henriksen J.R., King G.M., Belas R., Fuqua C., Brinkac L.M.,
RA Lewis M., Johri S., Weaver B., Pai G., Eisen J.A., Rahe E., Sheldon W.M.,
RA Ye W., Miller T.R., Carlton J., Rasko D.A., Paulsen I.T., Ren Q.,
RA Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R.,
RA Nelson W.C., Sullivan S.A., Rosovitz M.J., Haft D.H., Selengut J., Ward N.;
RT "Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine
RT environment.";
RL Nature 432:910-913(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 700808 / DSM 15171 / DSS-3;
RX PubMed=25780504; DOI=10.1186/1944-3277-9-11;
RA Rivers A.R., Smith C.B., Moran M.A.;
RT "An updated genome annotation for the model marine bacterium Ruegeria
RT pomeroyi DSS-3.";
RL Stand. Genomic Sci. 9:11-11(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000031; AAV94844.1; -; Genomic_DNA.
DR EMBL; CP000031; AAV96338.1; -; Genomic_DNA.
DR RefSeq; WP_011047294.1; NC_003911.12.
DR AlphaFoldDB; Q5LNV0; -.
DR SMR; Q5LNV0; -.
DR STRING; 246200.SPO1557; -.
DR EnsemblBacteria; AAV94844; AAV94844; SPO1557.
DR EnsemblBacteria; AAV96338; AAV96338; SPO3103.
DR KEGG; sil:SPO1557; -.
DR KEGG; sil:SPO3103; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_5; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000001023; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..558
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199374"
FT BINDING 67..74
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 558 AA; 60044 MW; FE0BD15E29A57DED CRC64;
MAYKSDIEIA REARKLPILE IGAKLGIAAD ELLPYGHDKA KVSQGFIDSV QDRADGRLIL
VTAINPTPAG EGKTTTTVGL GDGLNRIGKN AMICIREASL GPNFGMKGGA AGGGYAQIVP
MEEMNLHFTG DFHAITSAHS LLSAMIDNHI YWGNEADIDT RRVVWRRVVD MNDRALRQIT
CSLGGVSNGF PREAGFDITV ASEVMAILCL ARDLKDLEKR LGDIIVAYRR DKSPVYCRDI
KAEGAMTVLL KDAMQPNLVQ TLENNPAFVH GGPFANIAHG CNSVIATKTA LKVADYVVTE
AGFGADLGAE KFMNIKCRKA GIAPSAVVLV ATVRAMKMNG GVAKGDLGAE NVAAVNKGCA
NLGRHIENVK SFGVPVVVAI NHFVTDTDAE VQAVRDYCAN HGVEAVLSRH WELGSEGSEA
LARKVVELAE SGKANFAPIY PDDMSLFEKI ETIAKRIYRA DEVLADAKIR NQLKEWEEAG
YRNLPVCMAK TQYSFTTDPN RRGAPTGHSV PVREVRLSAG AGFIVVVCGE IMTMPGLPRK
PAAETIRLND AGQIEGLF
