ID   FTHS_SHEAM              Reviewed;         560 AA.
AC   A1SAE2;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Sama_3146;
OS   Shewanella amazonensis (strain ATCC BAA-1098 / SB2B).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=326297;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1098 / SB2B;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J.,
RA   Richardson P.;
RT   "Complete sequence of Shewanella amazonensis SB2B.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000507; ABM01349.1; -; Genomic_DNA.
DR   RefSeq; WP_011761253.1; NC_008700.1.
DR   AlphaFoldDB; A1SAE2; -.
DR   SMR; A1SAE2; -.
DR   STRING; 326297.Sama_3146; -.
DR   EnsemblBacteria; ABM01349; ABM01349; Sama_3146.
DR   KEGG; saz:Sama_3146; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000009175; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..560
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000293058"
FT   BINDING         62..69
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   560 AA;  59201 MW;  D41F2B8EC98CD7D6 CRC64;
     MLTDMEISRQ ANVRPIGWIA DDFGLGKHLW REAGHAMAKI SLEAARAPLR GKLVIVTAVT
     PTPHGEGKTV TTIGLTQGLH LLGHKACACI RQPSLGPVFG VKGGAAGGGY AQVVPMEVMN
     LHMTGDIHAV TSAHNLAAAA IDARLFHETR LGCESFSAKT GLPALNIDPE RILWRRVVDH
     NDRSLRQIEL GLGDNNGPVH GGGFDITAAS ELMAILALCE NIADMRARIG RVILAMSRDG
     KPISADDLGV AGAMTAIMRD AIEPTLMQTL SGAPCLVHAG PFANIAHGNS SIIADRVALA
     HHDYVVTEGG FGSDMGFEKF CNIKSRASGI APSAAVLVVT LRALKANSGV EGDDRARLEA
     GFANLHWHMQ NVMGYGLPLV VAINRFPDDA MAELDWLKER CLEEGAFGCE ISDAFSHGAE
     GARALATAVA RACDAPVNFE YLYDDEMDFG ARLMTLAERY GAAGVSLSED ARRDLAMITE
     LGMDKLPLCM AKTPLSISHD PSLKGAPSGF ELPVVGLRLS AGAGFITVLT GKVMTMPGLG
     LLPGYLNIDI NDKGEIIGLG