FTHS_SHEB2
ID FTHS_SHEB2 Reviewed; 580 AA.
AC B8ECE1;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Sbal223_3748;
OS Shewanella baltica (strain OS223).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=407976;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OS223;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Chertkov O., Meincke L., Brettin T.,
RA Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Ovchinnikova G., Brettar I., Rodrigues J., Konstantinidis K.,
RA Tiedje J.;
RT "Complete sequence of chromosome of Shewanella baltica OS223.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP001252; ACK48226.1; -; Genomic_DNA.
DR RefSeq; WP_012588639.1; NC_011663.1.
DR AlphaFoldDB; B8ECE1; -.
DR SMR; B8ECE1; -.
DR EnsemblBacteria; ACK48226; ACK48226; Sbal223_3748.
DR KEGG; sbp:Sbal223_3748; -.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002507; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..580
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000185262"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 580 AA; 60863 MW; 7A60C1B3357091C9 CRC64;
MLTDMDISSR ASLKNITELG ADLGLLPEEM MLFGHTKAKV ELSVLQRLAG QRKGKLIIVT
AVTPTPHGEG KTVTSIGLTQ SLNAIGQKAC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
EMNLHLTGDI HAVSSAHNLG AAAIAARLFH EARLGKTEFE AQSGQAFLDI APNEIRWHRV
VDHNDRCLRQ IHVGLGDNNG PEYGSSFDIT AASELMAILA LSHDLADMRA RIGRLVLALN
TQGQVITAED LGVAGAMTAI MADAIKPTLM QTLNGSPCLI HSGPFANIAH GNSSIIADDI
ALRLADFVVT EGGFGSDMGF EKFCNIKVRQ SGQAPAAAVL VTTLKALKAN SGLATEVDSN
APNIHVPNIN APDQARLEAG FANLNWHINN VARYGIPVVV GINRFATDSD AELQWLMEAV
NASAAFGCEI SDAFSQGEAG AIALARTVVR AAETESQFKL LYPDEASLEA KLSTLAEVGY
GASGVSLSIE AKQQAQQLTA LGYGHLPLCM AKTPLSISHD PSLKGVPKDF VVPVRELVLH
AGAGFITALV GNVMTMPGLG LKPGYLKIDI DAKGEIVGLG
