FTHS_SHEB8
ID FTHS_SHEB8 Reviewed; 585 AA.
AC A6WSY9;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Shew185_3804;
OS Shewanella baltica (strain OS185).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=402882;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OS185;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C.,
RA Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA Brettar I., Rodrigues J., Konstantinidis K., Tiedje J., Richardson P.;
RT "Complete sequence of chromosome of Shewanella baltica OS185.";
RL Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000753; ABS09928.1; -; Genomic_DNA.
DR RefSeq; WP_012090278.1; NC_009665.1.
DR AlphaFoldDB; A6WSY9; -.
DR SMR; A6WSY9; -.
DR KEGG; sbm:Shew185_3804; -.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..585
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000068794"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 585 AA; 61477 MW; 63780632AA87C3E9 CRC64;
MLTDMNISSR ASLKNITELG ADLGLLPEEM MLFGHTKAKV ELSVLQRLAG QRKGKLIIVT
AVTPTPHGEG KTVTSIGLTQ SLNAIGQKAC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
EMNLHLTGDI HAVSSAHNLG AAAIAARLFH EARLGKKEFE AQSGQAFLAI DPNEIRWHRV
VDHNDRCLRQ IHVGLGDNNG PEYESSFDIT AASELMAILA LSHDLADMRA RIGRLVLALN
TQGQVITADD LGVAGAMTAI MADAIKPTLM QTLNGSPCLI HSGPFANIAH GNSSIIADDI
ALRLADFVVT EGGFGSDMGF EKFCNIKVRQ SGQAPAAAVL VTTLKALKAN SGLATEVDSN
VSNIHVPNIN ATNINAPDQA RLEAGFANLN WHINNVARYG IPVVVAINRF ATDSDAELQW
LMEAVNASAA FGCEISDAFS QGEAGAIALA QTVVRAAETE SQFKLLYPDE VSLEAKLSTL
AEVGYGAAGV SLSIEAKQQA QQLTALGYGH LPLCMAKTPL SISHDPSLKG VPKDFVVPVR
ELVLHAGAGF ITALVGNVMT MPGLGLKPGY LKIDIDAKGE IVGLG
