FTHS_SHEB9
ID FTHS_SHEB9 Reviewed; 585 AA.
AC A9L3Z6;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Sbal195_3930;
OS Shewanella baltica (strain OS195).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=399599;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OS195;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Brettar I.,
RA Rodrigues J., Konstantinidis K., Klappenbach J., Hofle M., Tiedje J.,
RA Richardson P.;
RT "Complete sequence of chromosome of Shewanella baltica OS195.";
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000891; ABX51090.1; -; Genomic_DNA.
DR RefSeq; WP_006084234.1; NC_009997.1.
DR AlphaFoldDB; A9L3Z6; -.
DR SMR; A9L3Z6; -.
DR EnsemblBacteria; ABX51090; ABX51090; Sbal195_3930.
DR GeneID; 11774994; -.
DR KEGG; sbn:Sbal195_3930; -.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000770; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..585
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000087655"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 585 AA; 61417 MW; 74B1B8472E03015F CRC64;
MLTDMDISSR ASLKNITEIG VDLGLLPEEM MLFGHTKAKV ELSVLQRLAG QRKGKLIIVT
AVTPTPHGEG KTVTSIGLTQ SLNAIGQKAC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
EMNLHLTGDI HAVSSAHNLG AAAIAARLFH EARLGKTEFE AQSGQAFLDI APNEIRWHRV
VDHNDRCLRQ IHVGLGDNNG PEYGSSFDIT AASELMAILA LSHDLADMRV RIGRLVLALN
TQGQVITAED LGVAGAMTAI MADAIKPTLM QTLNGSPCLI HSGPFANIAH GNSSIIADDI
ALRLADFVVT EGGFGSDMGF EKFCNIKVRQ SGQAPAAAVL VTTLKALKAN SGLATEVDSN
VSNIHVPKIN AANINAPDQA RLEAGFANLN WHINNVARYG IPVVVAINRF ATDSDAELQW
LMEAVNASAA FGCEISDAFS QGEAGAIALA QTVVRAAEIE SQFKLLYPDE ASLEAKLSTL
AEVGYGAAGV SLSIEAKQQA QQLTALGYGH LPLCMAKTPL SISHDPSLKG VPKDFVVPVR
ELVLHAGAGF ITALVGNVMT MPGLGLKPGY LKIDIDAKGE IVGLG
