FTHS_SHEHH
ID FTHS_SHEHH Reviewed; 570 AA.
AC B0TVP3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Shal_3806;
OS Shewanella halifaxensis (strain HAW-EB4).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=458817;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HAW-EB4;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Kim E., Zhao J.-S., Richardson P.;
RT "Complete sequence of Shewanella halifaxensis HAW-EB4.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000931; ABZ78346.1; -; Genomic_DNA.
DR RefSeq; WP_012278864.1; NC_010334.1.
DR AlphaFoldDB; B0TVP3; -.
DR SMR; B0TVP3; -.
DR STRING; 458817.Shal_3806; -.
DR EnsemblBacteria; ABZ78346; ABZ78346; Shal_3806.
DR KEGG; shl:Shal_3806; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000001317; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..570
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000087656"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 570 AA; 60793 MW; 80790262CD6908E4 CRC64;
MQSDIEISRQ HTCLPITEIA KDLGLNPDEF SPCGVNKAKV SQSVARRLAD KKNAKLVIVT
AVTPTPFGEG KTVTTIGLTQ AMNLNGIKTC ACIRQPSMGP VFGTKGGAAG GGYAQVVPME
ELNLHLTGDI HAVSSAHNLA AAAIDARLFH EHRLGAEVFA QESGLSALNI DSENILWRRV
VDHNERSLRQ IKVGYGKVNG PVHESGFDIT AASELMAILA LSQDLKDLRQ RIGRLVLALN
TQGEPITAEE LGVAGAMTVI MADAIEPTLM QTLSGDPCFI HAGPFANIAH GNSSIIADTI
AAKLADVVIT EAGFGSDMGF EKFSNIKVRE SGYAPSASVV VVTLKALKAN SGIESDKDIN
QPDMERLQAG FANLEWHINN VSQYGVPVVV AINRFPTDTD EELEWLKQAV EQTKAFGSEI
SEAFGKGAEG ATKLADMVYH ATQTPSDFKL LYQSDTSLEA KLMTLAEVGY GASSVTLSDK
AKSQLHWLTK HNYGQLPICV AKTPMSISHD PSIKGIPTEF ELPITELRLN AGAGFVTALV
GQVMTMPGLG IKPGYLNVDI NDDGEIIGLA
