FTHS_SHEPA
ID FTHS_SHEPA Reviewed; 570 AA.
AC A8H8Z4;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Spea_3720;
OS Shewanella pealeana (strain ATCC 700345 / ANG-SQ1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=398579;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700345 / ANG-SQ1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C.,
RA Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA Zhao J.-S.Z., Manno D., Hawari J., Richardson P.;
RT "Complete sequence of Shewanella pealeana ATCC 700345.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000851; ABV89031.1; -; Genomic_DNA.
DR RefSeq; WP_012156913.1; NC_009901.1.
DR AlphaFoldDB; A8H8Z4; -.
DR SMR; A8H8Z4; -.
DR STRING; 398579.Spea_3720; -.
DR EnsemblBacteria; ABV89031; ABV89031; Spea_3720.
DR KEGG; spl:Spea_3720; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002608; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..570
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000087657"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 570 AA; 60496 MW; A5265CCF8F388332 CRC64;
MHSDIEISRQ HTCLPITDIA NNLGLEPDEL SLCGANKAKI SLSVAKRLAD RENAKLVIVT
AVTPTPFGEG KTVTTIGLTQ AMNLNGIKAC ACIRQPSMGP VFGTKGGAAG GGYAQVVPME
ELNLHLTGDI HAVSSAHNLA AAAIDARLFH ETRLGAEAFT QESGLTALNI DAENILWRRV
VDHNERSLRQ IKVGFGAVNG PVHESGFDIT AASELMAILA LSQDLKDLRQ RIGRLVLALN
NQGEPITAET LGVAGAMTVI MADAIEPTLM QTLSGDPCFI HAGPFANIAH GNSSIIADTI
AAKLADVVVT EAGFGSDMGF EKFSNIKVRE SGYAPSASVV VVTLKALKAN SGIESDQDIN
QPDTQRLKVG FANLEWHINN VSQYGVPVVV AINRFPTDTD EELEWLKQAI EQTKAFGSEI
SEAFGKGAEG ATKLADMVYR ATQTPSDFNL LYESNTSLES KLMTLAEVGY GASSVTLSDK
AKSQLNWLAK HNYGELPICV AKTPMSISHD PDIKGIPTGF ELPITELRLN AGAGFVTALV
GKVMTMPGLG IKPGYLNVDI NDDGEIVGLA
