ID   FTHS_SHEPC              Reviewed;         570 AA.
AC   A4YAP3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Sputcn32_3314;
OS   Shewanella putrefaciens (strain CN-32 / ATCC BAA-453).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=319224;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CN-32 / ATCC BAA-453;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Fredrickson J.,
RA   Tiedje J., Richardson P.;
RT   "Complete sequence of Shewanella putrefaciens CN-32.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000681; ABP77026.1; -; Genomic_DNA.
DR   RefSeq; WP_011920004.1; NC_009438.1.
DR   AlphaFoldDB; A4YAP3; -.
DR   SMR; A4YAP3; -.
DR   STRING; 319224.Sputcn32_3314; -.
DR   GeneID; 45043808; -.
DR   KEGG; spc:Sputcn32_3314; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   OMA; CGEIMTM; -.
DR   UniPathway; UPA00193; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..570
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000068795"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   570 AA;  60067 MW;  CDC0470D8964E0B9 CRC64;
     MLTDMEISSR ASLTNVAELG AELGLLPEEM LLFGSTKAKV DLSVQQRLAG QSRGKLIIVT
     AVTPTPHGEG KTVTSIGLTQ SLKAIGKKAC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
     ELNLHLTGDI HAVSSAHNLG AAAIAARLFH ESRLGKVEFE AQSGQAFLDI DPAQIRWHRV
     LDHNDRCLRQ VQVGLGENNG PVYESGFDIT AASELMAILA LSANLADMRA RIGRLVLALN
     RQGQVISAED LGVAGAMTAI MVDAIKPTLM QTLNGAPCLI HAGPFANIAH GNSSIIADDI
     ALKLVDYVIT EGGFGSDMGF EKFCNIKVRQ SGQVPSAAVL VTTLKALKAN SGIESDTDIN
     TPDEIRLEAG FANLHWHINN VARYGIPVVV AINRFATDTE AELQWLINAV NQTAAFGCEV
     SDAFSQGEAG AIELAHTVVR AADTQSQFRL LYPDDASLEA KLSTLAEVGY GAAGISLSDE
     AKQQVREFTS LGYAHLPVCM AKTPLSISHD PIQKGVPKDF IVPIRALVLN AGAGFVTALV
     GNVMTMPGLG IKPGYLKIDI DEAGEIIGLG