FTHS_SHEPW
ID FTHS_SHEPW Reviewed; 570 AA.
AC B8CI99;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=swp_0551;
OS Shewanella piezotolerans (strain WP3 / JCM 13877).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=225849;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WP3 / JCM 13877;
RX PubMed=18398463; DOI=10.1371/journal.pone.0001937;
RA Wang F., Wang J., Jian H., Zhang B., Li S., Wang F., Zeng X., Gao L.,
RA Bartlett D.H., Yu J., Hu S., Xiao X.;
RT "Environmental adaptation: genomic analysis of the piezotolerant and
RT psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans
RT WP3.";
RL PLoS ONE 3:E1937-E1937(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000472; ACJ27375.1; -; Genomic_DNA.
DR RefSeq; WP_020910756.1; NC_011566.1.
DR AlphaFoldDB; B8CI99; -.
DR SMR; B8CI99; -.
DR STRING; 225849.swp_0551; -.
DR EnsemblBacteria; ACJ27375; ACJ27375; swp_0551.
DR KEGG; swp:swp_0551; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000753; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..570
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000196823"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 570 AA; 60198 MW; 602AA4ADA64F3C18 CRC64;
MLSDINISRK HVCKPIAEIA TNLGIVESEL SVHGANKAKI SLSVTNRLAS KPDAKLVIVT
AVTPTPYGEG KTVTTVGLTQ AMNAIGVKTC ACIRQPSMGP VFGIKGGAAG GGYAQVVPME
ELNLHLTGDI HAVSSAHNLA AAAIDARLFH ETRLGAIAFT KESGLNAVNI DPENILWRRV
VDHNERSLRQ IEVGFGAVNG PVHSSGFDIT KASELMAILA LSRDVKDLRQ RVGRLVLALD
LVGKPITAED IGVAGAMTVI MRDAIEPTLM QTLSGDPCFI HAGPFANIAH GNSSIIADSI
ASKLADVVVT EAGFGSDMGF EKFSNIKVRE SGYKPSAAVV VVTLRALKAN SGIESEVAID
QPDMARLEAG FANLQWHINN VHQYGAPVVV AINRFPTDTS AELDWLQAKV ATTNAFGCAI
SDAFANGAQG AAELAKVVHA ATMVESEFNC LYETTDTLES KLMTLAEVGY GAASVSLSDK
AKLQLAWLHK QGYSELPVCI AKTPMSISHD PNVKGVPRNF ELPINELRLN AGAGFITALV
GKVMTMPGLG IKPGYLNIDI DADDEIIGLA
