FTHS_SHESA
ID FTHS_SHESA Reviewed; 570 AA.
AC A0KSN0;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN OrderedLocusNames=Shewana3_0560;
OS Shewanella sp. (strain ANA-3).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=94122;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ANA-3;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Newman D.,
RA Salticov C., Konstantinidis K., Klappenback J., Tiedje J., Richardson P.;
RT "Complete sequence of chromosome 1 of Shewanella sp. ANA-3.";
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000469; ABK46799.1; -; Genomic_DNA.
DR RefSeq; WP_011715760.1; NC_008577.1.
DR AlphaFoldDB; A0KSN0; -.
DR SMR; A0KSN0; -.
DR STRING; 94122.Shewana3_0560; -.
DR EnsemblBacteria; ABK46799; ABK46799; Shewana3_0560.
DR KEGG; shn:Shewana3_0560; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002589; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..570
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000300538"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 570 AA; 59643 MW; E48879BB808BCCBA CRC64;
MLTDMDISRR AHLKDIAALG AEFGLLPDEM QLFGKTKAKV DLRAQQRLAE QQQGKLIIVT
AVTPTPHGEG KTVTTIGLTQ SLKALGNKVC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
ELNLHLTGDI HAVSSAHNLG AAAIASRLYH ETRLGKAEFE SQSGQNYLDI APNGIRWHRV
VDHNDRCLRE IEVGLGENNG PAYTSGFDIT AASELMAILA LSRNLADMRA RIGKLVLAVN
RQGAAISAED LGVAGAMTAI MADAVKPTLM QTLNGAPCLI HAGPFANIAH GNSSVIADDI
ALKLADFVVT EGGFGSDMGF EKFCNIKARQ SGLAPSAAVL VTTLKALKAN SGLTSDADIN
APDQARLEAG FANLNWHINN VARYGIPVVV AINRFATDTD AELNWLIEAV AGTAAFGCEL
SEAFSQGEAG ALTLAQTVMR ACEQPSEFTL LYPDEMALEA KLSTLAEVGY GATGVSLSEA
AKLQLQELSA LGYADLPVCM AKTPLSISHD PQLKGVPQGF IVPVRELVLN AGAGFITALV
GNVMTMPGLG LVPGYLKVDI GADGEITGLG
