FTHS_SHEWM
ID FTHS_SHEWM Reviewed; 570 AA.
AC B1KJC0;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Swoo_4339;
OS Shewanella woodyi (strain ATCC 51908 / MS32).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=392500;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51908 / MS32;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Lykidis A., Zhao J.-S., Richardson P.;
RT "Complete sequence of Shewanella woodyi ATCC 51908.";
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000961; ACA88592.1; -; Genomic_DNA.
DR RefSeq; WP_012326918.1; NC_010506.1.
DR AlphaFoldDB; B1KJC0; -.
DR SMR; B1KJC0; -.
DR STRING; 392500.Swoo_4339; -.
DR EnsemblBacteria; ACA88592; ACA88592; Swoo_4339.
DR KEGG; swd:Swoo_4339; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002168; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..570
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000196824"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 570 AA; 60642 MW; 8596D91447E9335C CRC64;
MLTDIEISRQ ATPQPISDIA KKFGIKKDEL SQFGDAKAKV KISILDRVKD NVEGKLVIVT
AVTPTPFGEG KTVTSIGLTQ GLNAIGRKTC ACIRQPSMGP VFGIKGGAAG GGYSQVIPME
EMNLHLTGDI HAVSSAHNLA AAAVDARLFH ESRLSADEYT LQSGSAPLNI DPEQILWRRV
VDHNERSLRN ITVGLGDING PVHASGFDIT AASELMAILA LSHDLKDMRK RIGRLVLALD
KHAQPITAEM IGVAGAMTVI MSNAIEPTLM QTLTGDPCLI HAGPFANIAH GNSSIIADRI
ALKFADFVVT EGGFGSDMGF EKFCNIKVRE SGKAPDAAVL VVTLKALKAN SGLESKQDIN
LPDQARLEAG FLNLKWHMDN VSQYGVPVVV ALNRFPSDTQ EELEWLKREV MLAGAFGCEI
SDAFSLGANG AEALAHKVVE ATEQDSDFRL LYSSDASIEA KLLTIAESGY GARGVVLSDE
AKAQLEQIKA MGLDNLAVCI AKTPLSISHD PLLKGVPQGF ELPIAQLKIN AGAGFITALV
GKVMTMPGLG VKPGYLNIDI NEQDEIVGLA
