ID   FTHS_SINMW              Reviewed;         567 AA.
AC   A6UBV0;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Smed_2298;
OS   Sinorhizobium medicae (strain WSM419) (Ensifer medicae).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=366394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WSM419;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G.,
RA   Richardson P.;
RT   "Complete sequence of Sinorhizobium medicae WSM419 chromosome.";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000738; ABR61130.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6UBV0; -.
DR   SMR; A6UBV0; -.
DR   STRING; 366394.Smed_2298; -.
DR   EnsemblBacteria; ABR61130; ABR61130; Smed_2298.
DR   KEGG; smd:Smed_2298; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_5; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001108; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..567
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000318569"
FT   BINDING         76..83
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   567 AA;  61461 MW;  112175D55AA5B11A CRC64;
     MRKQLGEAVG EVKTDIEIAR AARKQPIMEV GARLGIPPEH LLPYGHDKAK VSAEFIAAQR
     EKKNGRLILV TAINPTPAGE GKTTTTVGLV DGLNRIGKKA IVCIREASLG PCFGIKGGAA
     GGGYAQVVPM EDINLHFTGD FHAITSAHNL LSALIDNHIY WGNEQAIDIR RIAWRRVMDM
     NDRALRHIVG SLGGVANGYP RETGFDITVA SEVMAILCLA MDIRDLERRL GNIIIGYRRD
     KSPVYARDIK ADGAMAVLLK DAMQPNLVQT LENNPAFVHG GPFANIAHGC NSVVATTTAL
     KLADYVVTEA GFGADLGAEK FFDIKCRKAG LMPDAAVIVA TVRAIKMNGG VKKEDLAKEN
     VEALRKGCPN LGRHIQNVKK FGVPVLVAIN HFTSDTEAEI QAIKDYVRTL GSEAVLCKHW
     AEGSAGIEEL ADKVADLADA GHSQFSPLYP DEMPLFQKIE AIAKDIYHAS EVIADKLVRD
     QLRIWEDQGY GHLPICMAKT QYSFSTDPNL RGAPTGHTVP IREVRLAAGA GFIVVITGEI
     MTMPGLPKVP SSERIRLDEE GYIEGLF