FTHS_STACT
ID FTHS_STACT Reviewed; 555 AA.
AC B9DN77;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Sca_1337;
OS Staphylococcus carnosus (strain TM300).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=396513;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TM300;
RX PubMed=19060169; DOI=10.1128/aem.01982-08;
RA Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C.,
RA Goetz F.;
RT "Genome analysis of the meat starter culture bacterium Staphylococcus
RT carnosus TM300.";
RL Appl. Environ. Microbiol. 75:811-822(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; AM295250; CAL28242.1; -; Genomic_DNA.
DR RefSeq; WP_015900582.1; NC_012121.1.
DR AlphaFoldDB; B9DN77; -.
DR SMR; B9DN77; -.
DR STRING; 396513.SCA_1337; -.
DR KEGG; sca:SCA_1337; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_9; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR BioCyc; SCAR396513:SCA_RS06650-MON; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000444; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..555
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000185263"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 555 AA; 59962 MW; E66582F8793796D2 CRC64;
MTHLSDLDIA NQATIKPISE IAEKIGIPED ALEQYGHYKA KIDINKLDDK GDRGKVVLVT
AMSPTPAGEG KSTVTVGLAD AFHELGENVM MALREPALGP VFGIKGGATG GGYAQVLPME
DINLHFNGDF HAITTANNAL AAFIDNHIHQ GNELGIDQRR IEWKRVLDMN DRELRKVVVG
LGGPTQGVPR EDGFNITVAS EIMTILCLST GLKDLKASIA NITIGYTRDR KPVTVADLKV
EGALAMILKD AIKPNLVQSI EGTPALIHGG PFANIAHGCN SIIATETARK LADIVVTEAG
FGSDLGAEKF MNIKARKAGF EPSAAVVVAT IRALKMHGGV AKDDLKEENV QAVRDGLANL
ERHIENIRSF GVEPVVALNA FVSDTEAEEQ VVEDWAKEHG VRIALTEVWE KGGKGGVELA
KQVQEVLNEK HDFKHTYDLD LPIEEKIEKV VTNIYGGNKV TFTSGALKQL KQIKENGWDN
YPVCMAKTQY SFTDDKDRLG APDDFEITIR ELQPKTGAGF IVALTGAIMT MPGLPKKPAA
LNMDVTEDGH AKGLF
