FTHS_SYMTH
ID FTHS_SYMTH Reviewed; 556 AA.
AC Q67JH9;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=STH3189;
OS Symbiobacterium thermophilum (strain T / IAM 14863).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Symbiobacteriaceae;
OC Symbiobacterium.
OX NCBI_TaxID=292459;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T / IAM 14863;
RX PubMed=15383646; DOI=10.1093/nar/gkh830;
RA Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K.,
RA Ikeda H., Hattori M., Beppu T.;
RT "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium
RT that depends on microbial commensalism.";
RL Nucleic Acids Res. 32:4937-4944(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; AP006840; BAD42171.1; -; Genomic_DNA.
DR RefSeq; WP_011197302.1; NC_006177.1.
DR AlphaFoldDB; Q67JH9; -.
DR SMR; Q67JH9; -.
DR STRING; 292459.STH3189; -.
DR EnsemblBacteria; BAD42171; BAD42171; STH3189.
DR KEGG; sth:STH3189; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_9; -.
DR OMA; TRQGFSK; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000417; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..556
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199401"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 556 AA; 59450 MW; 49822EE6AAD9BDAE CRC64;
MLSDIAIAQR ARLEPITKVA EQIGLGPEDL ELYGRYKAKV ADHVWPRVRS NPDGKLILVT
AISPTPAGEG KTTVTVGLGQ AMSRIGKRAI IALREPSLGP AFGVKGGAAG GGYSQVVPMD
EINLHFTGDF HAVTAANNLL AAMIDNHLHQ GNKLGLDPRQ ITFKRVLDMN DRALRSVVIG
LGGKNGGVPR QEEFMITPAS EVMATLCLAE DLADLKRRCG EIIVGYTYDG APVRARDLKA
EGAMATLLKE AIKPNLVQTL ENTPAFVHGG PFANIAHGCN TVVATRLALK LADYVITEAG
FGADLGAEKF IDIKCRLSGL RPDAVVVVAT IRSLKMHGGL KKDDLAREDL EALQRGSANL
MRHLRNVTEV FGLPAVVAIN RFAADTEAEI ALLRALVEEA GATAVVADVH ARGGDGGIEL
AQRVVELVEQ PNRFRFAYDD EDSLKTKIEK VATRIYGADG VDFTREASRM LKKLESEGFG
RAPVCIAKTQ YSFSDDPKKL GAPTGWRLTV REVRPSAGAG FVVALTGEIM TMPGLPPVPA
AESIDVSDDG EITGLF
