FTHS_THEAC
ID FTHS_THEAC Reviewed; 536 AA.
AC Q9HI67;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Ta1478;
OS Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC
OS 15155 / AMRC-C165).
OC Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC Thermoplasmataceae; Thermoplasma.
OX NCBI_TaxID=273075;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165;
RX PubMed=11029001; DOI=10.1038/35035069;
RA Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C.,
RA Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.;
RT "The genome sequence of the thermoacidophilic scavenger Thermoplasma
RT acidophilum.";
RL Nature 407:508-513(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; AL445067; CAC12596.1; -; Genomic_DNA.
DR RefSeq; WP_010901878.1; NC_002578.1.
DR AlphaFoldDB; Q9HI67; -.
DR SMR; Q9HI67; -.
DR STRING; 273075.Ta1478; -.
DR EnsemblBacteria; CAC12596; CAC12596; CAC12596.
DR GeneID; 1456930; -.
DR KEGG; tac:Ta1478; -.
DR eggNOG; arCOG04541; Archaea.
DR HOGENOM; CLU_003601_3_3_2; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 15172at2157; -.
DR BRENDA; 6.3.4.3; 6324.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000001024; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..536
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199415"
FT BINDING 51..58
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 536 AA; 57647 MW; 82D0405ABA148E7C CRC64;
MRDIEEIIKE IGLGRDDYEL YGRYMAKLDL GLQNRGRPRA KLILVTAMTP TPAGEGKTTT
TIGLGQAMKK LGKNVAIAIR EPSLGPCFGI KGGATGGGKS KVEPSDRINL FFTGDFPTIT
AAHNLLSAMI NNHMYHGNEL NLDPKRITFP RTIDMNDRSL RSILVGVGPR DMGVLAGDSF
VITPASEVMA ITGLSLNYQD LKARLSRILA GFTTKNKPVF AGDLKAEGSM AALLRDALKP
NLVQTTEGVP AFVHTGPFGN IAHGTSSLVA DKIALNLFDY VITEAGFGSD LGAEKFFNLA
SRIGDLPINA VVLVATIRAI KHHGGSKKEG EDIDAVKTGS KNLIRHVQNI RNFGIDPVVA
VNRFPTDTDG EIRALGEILD SNGIKWALSE VFAKGGEGGI DLANKVLASL GSHEIKRTYD
LKDDIRTKIE KIARNVYGAD GVIFEKKALS DMKKAEEIME NPYVCMAKTQ YSFSDDASLL
NDPHGFTIKV QSINISSGAG FVVPILGEIM TMPGLPKHPA AENIDLTDSG EITGLF
