ID   FTHS_THEM4              Reviewed;         551 AA.
AC   A6LJS5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Tmel_0304;
OS   Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429).
OC   Bacteria; Thermotogae; Thermotogales; Fervidobacteriaceae; Thermosipho.
OX   NCBI_TaxID=391009;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 12029 / CIP 104789 / BI429;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C.,
RA   Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.;
RT   "Complete sequence of Thermosipho melanesiensis BI429.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000716; ABR30176.1; -; Genomic_DNA.
DR   RefSeq; WP_012056537.1; NC_009616.1.
DR   AlphaFoldDB; A6LJS5; -.
DR   SMR; A6LJS5; -.
DR   STRING; 391009.Tmel_0304; -.
DR   EnsemblBacteria; ABR30176; ABR30176; Tmel_0304.
DR   KEGG; tme:Tmel_0304; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_0; -.
DR   OMA; CGEIMTM; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001110; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..551
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000068798"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   551 AA;  59916 MW;  1ACF712B6DCC63BF CRC64;
     MKTDIEIARE AKLKRISDIA KDIGIEEKYL EPYGKYIAKV DLKFLKTLNN KKDGKLILVT
     AITPTPAGEG KTTTSIGLSM ALNRLGKKSI VTLREPSLGP VFGIKGGAAG GGYSQVLPME
     NINLHFTGDI HAISSAHNLI SAVIDSHIKF GNNLNINPTK VFWKRTMDMN DRALRQIIVG
     LGGNANGLPR EDGFIITAAS EIMAILCLAK DLKDLKERLG NIVVAESFDK KLVRVKDLKI
     EGALTALLKD AINPNIVQTI ENTPAFIHGG PFANIAHGTN SIIATKLALK LTDYVVTEAG
     FAADLGAEKF LDFVTQVGNF DVNAVVLVAT IKALKYHGGI KKDELNKENV DAMLKGMENL
     KTHIENLKLY NVPVVVALNV FASDTTNELK AFSEHCNAPH ALVRAFEKGS KGTIELANTV
     LENISDSKHI PILNPEMSIE EKIETLATKV YRARKVIYTD TAKSKLKFLK RHGFETLPVI
     VAKTQSSISD DPKKLNAPRD YNFTIRDFEL SAGAGFVVAL AGEIMRMPGL SKIPNAVNID
     VDEDGNIVGL S