FTHS_THEMA
ID FTHS_THEMA Reviewed; 542 AA.
AC Q9X287;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=TM_1766;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; AE000512; AAD36830.1; -; Genomic_DNA.
DR PIR; E72212; E72212.
DR RefSeq; NP_229563.1; NC_000853.1.
DR RefSeq; WP_010865397.1; NZ_CP011107.1.
DR PDB; 3DO6; X-ray; 1.85 A; A/B=1-542.
DR PDBsum; 3DO6; -.
DR AlphaFoldDB; Q9X287; -.
DR SMR; Q9X287; -.
DR STRING; 243274.THEMA_05395; -.
DR EnsemblBacteria; AAD36830; AAD36830; TM_1766.
DR KEGG; tma:TM1766; -.
DR eggNOG; COG2759; Bacteria.
DR InParanoid; Q9X287; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR EvolutionaryTrace; Q9X287; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Ligase; Nucleotide-binding;
KW One-carbon metabolism; Reference proteome.
FT CHAIN 1..542
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199403"
FT BINDING 53..60
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
FT HELIX 4..10
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 15..17
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 18..21
FT /evidence="ECO:0007829|PDB:3DO6"
FT TURN 22..24
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 25..28
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 32..35
FT /evidence="ECO:0007829|PDB:3DO6"
FT TURN 36..38
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 43..51
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 59..72
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 77..81
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 86..91
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 102..106
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 107..111
FT /evidence="ECO:0007829|PDB:3DO6"
FT TURN 112..115
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 117..137
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 144..149
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 153..157
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 160..162
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 163..168
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 172..174
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 178..180
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 182..184
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 185..187
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 189..196
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 200..208
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 211..215
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 220..222
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 223..226
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 229..235
FT /evidence="ECO:0007829|PDB:3DO6"
FT TURN 236..240
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 243..247
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 252..255
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 261..264
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 270..279
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 281..290
FT /evidence="ECO:0007829|PDB:3DO6"
FT TURN 291..293
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 294..300
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 302..306
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 311..317
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 319..325
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 330..332
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 338..357
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 362..367
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 374..385
FT /evidence="ECO:0007829|PDB:3DO6"
FT TURN 386..388
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 390..394
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 396..399
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 400..403
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 405..414
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 430..440
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 445..449
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 451..462
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 470..473
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 476..481
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 493..496
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 498..502
FT /evidence="ECO:0007829|PDB:3DO6"
FT TURN 503..506
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 507..511
FT /evidence="ECO:0007829|PDB:3DO6"
FT HELIX 526..529
FT /evidence="ECO:0007829|PDB:3DO6"
FT STRAND 538..540
FT /evidence="ECO:0007829|PDB:3DO6"
SQ SEQUENCE 542 AA; 58538 MW; BC3AE16461AF326E CRC64;
MKPIKEIADQ LELKDDILYP YGHYIAKIDH RFLKSLENHE DGKLILVTAV TPTPAGEGKT
TTSIGLSMSL NRIGKKSIVT LREPSLGPTL GLKGGATGGG RSRVLPSDEI NLHFTGDMHA
VASAHNLLAA VLDSHIKHGN ELKIDITRVF WKRTMDMNDR ALRSIVIGLG GSANGFPRED
SFIITAASEV MAILALSENM KDLKERLGKI IVALDADRKI VRISDLGIQG AMAVLLKDAI
NPNLVQTTEG TPALIHCGPF ANIAHGTNSI IATKMAMKLS EYTVTEAGFG ADLGAEKFID
FVSRVGGFYP NAAVLVATVR ALKYHGGANL KNIHEENLEA LKEGFKNLRV HVENLRKFNL
PVVVALNRFS TDTEKEIAYV VKECEKLGVR VAVSEVFKKG SEGGVELAKA VAEAAKDVEP
AYLYEMNDPV EKKIEILAKE IYRAGRVEFS DTAKNALKFI KKHGFDELPV IVAKTPKSIS
HDPSLRGAPE GYTFVVSDLF VSAGAGFVVA LSGDINLMPG LPKKPNALNM DVDDSGNIVG
VS
