ID   FTHS_THESQ              Reviewed;         542 AA.
AC   B1LAQ7;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=TRQ2_1058;
OS   Thermotoga sp. (strain RQ2).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga;
OC   unclassified Thermotoga.
OX   NCBI_TaxID=126740;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RQ2;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D.B., Goodwin L., Pitluck S., Saunders E., Brettin T.,
RA   Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K.,
RA   Richardson P.;
RT   "Complete sequence of Thermotoga sp. RQ2.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000969; ACB09405.1; -; Genomic_DNA.
DR   RefSeq; WP_012310915.1; NC_010483.1.
DR   AlphaFoldDB; B1LAQ7; -.
DR   SMR; B1LAQ7; -.
DR   EnsemblBacteria; ACB09405; ACB09405; TRQ2_1058.
DR   KEGG; trq:TRQ2_1058; -.
DR   HOGENOM; CLU_003601_3_3_0; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001687; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..542
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000146708"
FT   BINDING         53..60
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   542 AA;  58727 MW;  AB6E352402AEF6C5 CRC64;
     MKPIKEIADQ LGLKDDVLYP YGHYIAKIDH RFLKSLENRE DGKLILVTAV TPTPAGEGKT
     TTSIGLSMSL NRIGKKSIVT LREPSLGPTL GLKGGATGGG RSRVLPSDEI NLHFTGDMHA
     VASAHNLLAA VLDSHIKHGN ELKIDITRVF WKRTMDMNDR ALRSIVIGLG GSANGFPRED
     SFIITAASEV MAVLALSENM KDLKERLGKI IVALNTDRKI VRVSDLRIQG AMAVLLKDAI
     NPNLVQTTEG TPALIHCGPF ANIAHGTNSI IATKMAMKLS EYTVTEAGFG ADLGAEKFID
     FVSRVGGFYP NAAVLVATVR ALKYHGGADL KNIHEENLEA LKEGFKNLRV HLENLRKFNL
     PVVVALNRFI TDTEKEIAYV VKECEKLGVR VAVSEVFEKG SEGGVELAKA VTEAVKDVKP
     VYLYEMNDPV EKKIEILAKE IYRAGRVEFS DTAKNALKFI KKHGFDELPV IVAKTPKSIS
     HDPSLRGAPE GYTFVVSDLF VSAGAGFVVA LSGDINLMPG LPERPNALNM DVDDSGNIVG
     VS