ID   FTHS_THEYD              Reviewed;         589 AA.
AC   B5YJE1;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=THEYE_A0513;
OS   Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87).
OC   Bacteria; Nitrospirae; Thermodesulfovibrionia; Thermodesulfovibrionales;
OC   Thermodesulfovibrionaceae; Thermodesulfovibrio.
OX   NCBI_TaxID=289376;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51303 / DSM 11347 / YP87;
RA   Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.;
RT   "The complete genome sequence of Thermodesulfovibrio yellowstonii strain
RT   ATCC 51303 / DSM 11347 / YP87.";
RL   Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP001147; ACI21937.1; -; Genomic_DNA.
DR   RefSeq; WP_012546634.1; NC_011296.1.
DR   RefSeq; YP_002248356.1; NC_011296.1.
DR   AlphaFoldDB; B5YJE1; -.
DR   SMR; B5YJE1; -.
DR   STRING; 289376.THEYE_A0513; -.
DR   EnsemblBacteria; ACI21937; ACI21937; THEYE_A0513.
DR   KEGG; tye:THEYE_A0513; -.
DR   PATRIC; fig|289376.4.peg.507; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_0; -.
DR   InParanoid; B5YJE1; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000718; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..589
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000146709"
FT   BINDING         74..81
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   589 AA;  64990 MW;  C8FACC5770FB32D5 CRC64;
     MLPEPVSCPD WLIAQEAEKR MKSIFQLAEE LDIKKEELIP YGSYIAKIDY RKLYSRIKNN
     PNGKYIIVTA ITPTPFGEGK STTTIGLTQG LGKRGKKVSC AIRQPSAGPL MNIKGSAAGG
     GLSQCIPRTE FSLGFTGDIN AVMNAHNLAM VALTSRMLHE ANYSDEILKK RGLRRLDIDP
     KRVQMGWVID FCVQALRKIV IGLGGKKDGI TMESRFDIAT SSELMAILSL VKDLKELKKR
     ISSIVVAYSK TGNPITTEDL EVSGAMSALM LPAFNPNLIQ TIEGQPVFVH AAPFANIAIG
     QSSIIADMVG LKLNEYHVTE CGFGADIGFE KFWNIKCRTS GLKPDVAVLV ATLRALKYHG
     ADKDSPKIIP GNPLPKEYIE KNMQWLERGM KNLFHHIKII KKSGLSIVVC INKFQSDTHE
     ELDFVRKFCE EMGIPVAISE HWQKGGQGAL ELADFVIDAC KNNSNFNFLY ENSLPHISRI
     ELIAREIYGA DSVEFSPLAL EKLQSINSKK EFSDFAICIA KTHLSLSDNP LLRGVPEGWQ
     LFIRDILVFY GAKLIVPVAG EISLMPGTAS TPNFRTIDVD LQTGKVTGI