ID   FTHS_UREPA              Reviewed;         521 AA.
AC   Q9PQS1;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; Synonyms=fthS;
GN   OrderedLocusNames=UU221;
OS   Ureaplasma parvum serovar 3 (strain ATCC 700970).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Ureaplasma.
OX   NCBI_TaxID=273119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700970;
RX   PubMed=11048724; DOI=10.1038/35037619;
RA   Glass J.I., Lefkowitz E.J., Glass J.S., Heiner C.R., Chen E.Y.,
RA   Cassell G.H.;
RT   "The complete sequence of the mucosal pathogen Ureaplasma urealyticum.";
RL   Nature 407:757-762(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AF222894; AAF30629.1; -; Genomic_DNA.
DR   RefSeq; WP_006688872.1; NC_002162.1.
DR   AlphaFoldDB; Q9PQS1; -.
DR   SMR; Q9PQS1; -.
DR   STRING; 273119.UU221; -.
DR   EnsemblBacteria; AAF30629; AAF30629; UU221.
DR   GeneID; 29672511; -.
DR   KEGG; uur:UU221; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_14; -.
DR   OMA; CGEIMTM; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000423; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..521
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199406"
SQ   SEQUENCE   521 AA;  58466 MW;  F2C5D80295186092 CRC64;
     MAKIIEEMIN KKGLLIDDYE LYGNEIAKIK SIKKLNKKDS KLIVITSMNP NPAGEGKTTT
     AIGLVDALNK HGYKAIGALR EPSMGPVFGM KGTGSGGGLS FLKPFDKINL HFSGDFHAIT
     AANNLIVAVI ENEIHNRSSM QIDSQKILIK RCLDVNDRSL RNIEYDINHQ QTKSGFNITA
     ASDLMALFCL AHDHKDFEDK LAQTIVAYNI VNQPIRICDL ELTKAIMAIL EDALYANLVR
     TNEDNPVFVH GGPFANIAHG CNSIIATKNA LALGDYVVTE CGFGSDLGLE KFMNIKMASL
     NLKPDLIGLV ISLKSIAYHA QTNEKDYVKQ GFANVLCHIN HIKKYNVSFI VYINVNTNTD
     SEEDLLTLEK LLDEHQIEHA RSYAYSYGSK KSEEITKKTI TLTNQINDHE LKLIYDIKDH
     LSYKLKKICE NVYGADGYEL SYEAKEQLNR YEHLDFYLCI AKTPYSISDD AKLLNNPKNF
     KIHIERFEIN YAAKLIIAIT TTIYRMPGLN KEPAAKNFVM K