ID   FTHS_VIBC1              Reviewed;         582 AA.
AC   A7N5E9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=VIBHAR_05943;
OS   Vibrio campbellii (strain ATCC BAA-1116).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=2902295;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1116 / BB120;
RG   The Vibrio harveyi Genome Sequencing Project;
RA   Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C.,
RA   Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K.,
RA   Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J.,
RA   Wilson R.K.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000790; ABU73836.1; -; Genomic_DNA.
DR   RefSeq; WP_012129485.1; NC_022270.1.
DR   AlphaFoldDB; A7N5E9; -.
DR   SMR; A7N5E9; -.
DR   EnsemblBacteria; ABU73836; ABU73836; VIBHAR_05943.
DR   KEGG; vha:VIBHAR_05943; -.
DR   PATRIC; fig|338187.25.peg.4351; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000008152; Chromosome II.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..582
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000068800"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   582 AA;  62330 MW;  2085192FBAF56CFB CRC64;
     MQSDIEICRN TPLSSIATVA ANAGLLPHEL DTHGKHKAKV HPQCLERLTD NQDGKLVLVT
     AITPTPLGEG KTVTTIGLSQ GLSKINQSVM ACIRQPSMGP VFGVKGGAAG GGYSQVAPMD
     ELNLHLTGDI HAVTAAHNLA SAALDARLYH EQREGYDAFE ARTSLRALKI DVERITWKRV
     MDHNDRALRM VKIGLNEEGK NINGFEREEG FDISAASELM AILALANDLK DLRQRIGRIV
     VAYDLNGNPV TTEDLQVAGA MAVTLKETIA PTLMQTLEGV PTLIHAGPFA NIAHGNSSII
     ADQIALKLSS YVVTEAGFGS DMGFEKACNI KALAADRAPD CVVIVATLRG LKANSGHYDL
     RPGMAIPDSI FNPDQAALEA GFENLKWHIN NVHKYGIPAV VAINQFPQDS GQELAELKAL
     IERFNPEVKV AVSTVFAQGG EGAIELAQHV VETCEQGAQF RPLYTKKQSL KEKLMAVCEV
     GYGASNIEMS ELAKQQLAHF EKLGFDDLAV CIAKTPLSIT TDSAIKGAPS GFAVPIRELR
     LCAGAGFVYA LSGNVMTMPG LPDKPTFMNL DLDDQGNIIG LS