FTHS_VIBVU
ID FTHS_VIBVU Reviewed; 582 AA.
AC Q8D7D8; Q8D7D9;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=VV2_0216;
GN ORFNames=VV2_0217;
OS Vibrio vulnificus (strain CMCP6).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=216895;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CMCP6;
RA Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP SEQUENCE REVISION.
RC STRAIN=CMCP6;
RX PubMed=21245845; DOI=10.1038/msb.2010.115;
RA Kim H.U., Kim S.Y., Jeong H., Kim T.Y., Kim J.J., Choy H.E., Yi K.Y.,
RA Rhee J.H., Lee S.Y.;
RT "Integrative genome-scale metabolic analysis of Vibrio vulnificus for drug
RT targeting and discovery.";
RL Mol. Syst. Biol. 7:460-460(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; AE016796; AAO07187.2; -; Genomic_DNA.
DR RefSeq; WP_011081194.1; NC_004460.2.
DR AlphaFoldDB; Q8D7D8; -.
DR SMR; Q8D7D8; -.
DR EnsemblBacteria; AAO07187; AAO07187; VV2_0216.
DR KEGG; vvu:VV2_0216; -.
DR HOGENOM; CLU_003601_3_3_6; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002275; Chromosome 2.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..582
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199410"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 582 AA; 62201 MW; 25EDDDE568C8D299 CRC64;
MLSDIDICRT TPLKPIEQIA AKAGLLADEF ETHGLYKAKV SARCVKRLAE QQDGKLVLVT
AITPTPLGEG KTVTTIGLAQ GLQSLNQSVM ACIRQPSMGP VFGVKGGAAG GGYSQVAPMD
ELNLHLTGDI HAVTAAHNLA AAAIDARIYH EQRAGYQAFE ARTGLPALRI DPDRVVWKRV
MDHNDRALRK VRVGINDEGK TINGFEREEG FDISAASELM AILALSKNLQ DMRERIGKVV
LAYNLDSEPV SAEQLQVAGA MTVTLKEAIK PTLMQTLEGV PTLIHAGPFA NIAHGNSSII
ADEIALKLSS YVVTEAGFGS DMGLEKACNI KSSTSHKAPD CVVIVATLRG LKANSGLYDL
KPGMPLPDAL FQPDRAALES GFSNLKWHID NAQKYGLPVV VAINQFPQDS AEELGQLKLW
IQQLPLNVRV AISEGFAKGG KGMEAVAREV IEACDAPANF RPLYRAEQTL EEKIITVCTK
GYGCGEVQFS DTAKQQLALY QTLGFGELAV CMAKTPLSIS SDSSLKGAPS GFTIMVREVR
LCAGAGFVYA LTGNVMTMPG LPDIPAFMAL DIDENGDIVG LS