FTHS_VIBVY
ID FTHS_VIBVY Reviewed; 582 AA.
AC Q7MEE8;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=VVA0722;
OS Vibrio vulnificus (strain YJ016).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=196600;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YJ016;
RX PubMed=14656965; DOI=10.1101/gr.1295503;
RA Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L.,
RA Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P.,
RA Lee C.-T., Hor L.-I., Tsai S.-F.;
RT "Comparative genome analysis of Vibrio vulnificus, a marine pathogen.";
RL Genome Res. 13:2577-2587(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; BA000038; BAC96748.1; -; Genomic_DNA.
DR RefSeq; WP_011152059.1; NC_005140.1.
DR AlphaFoldDB; Q7MEE8; -.
DR SMR; Q7MEE8; -.
DR STRING; 672.VV93_v1c37180; -.
DR EnsemblBacteria; BAC96748; BAC96748; BAC96748.
DR KEGG; vvy:VVA0722; -.
DR PATRIC; fig|196600.6.peg.3917; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002675; Chromosome II.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..582
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199411"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 582 AA; 62143 MW; BB203316FAE0BF06 CRC64;
MLSDIDICRT TPLKPIEQIA AKAGLLADEF ETHGLYKAKV SARCVKRLAE QQDGKLVLVT
AITPTPLGEG KTVTTIGLAQ GLQSLNQSVM ACIRQPSMGP VFGVKGGAAG GGYSQVAPMD
ELNLHLTGDI HAVTAAHNLA AAAIDARIYH EQRAGYQAFE ARTGLPALRI DPDRVVWKRV
MDHNDRALRK VRVGLNDEGK TINGFEREEG FDISAASELM AILALSKNLQ DMRERIGKVV
LAYNLDGEPV SAEQLQVAGA MTVTLKEAIK PTLMQTLEGV PTLIHAGPFA NIAHGNSSII
ADEIALKLSS YVVTEAGFGS DMGLEKACNI KSSTSHKAPD CVVIVATLRG LKANSGLYDL
KPGMPLPDAL FQPDRAALES GFSNLKWHID NAQKYGLPAV VAINQFPQDS AEELGQLKLW
IQQLPLNVRV AISEGFAKGG KGMEAVAREV IEACDAPANF RPLYRAEQTL EEKIITVCTK
GYGCGEVQFS DTAKQQLALY QTLGFGELAV CMAKTPLSIS SDSSLKGAPS GFTIMVREVR
LCAGAGFVYA LTGNVMTMPG LPDIPAFMAL DIDENGDIVG LS