ID   FTR_METCA               Reviewed;         298 AA.
AC   Q9ADT1; Q603E9;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 2.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Formylmethanofuran--tetrahydromethanopterin formyltransferase {ECO:0000255|HAMAP-Rule:MF_00579};
DE            Short=Ftr {ECO:0000255|HAMAP-Rule:MF_00579};
DE            EC=2.3.1.101 {ECO:0000255|HAMAP-Rule:MF_00579};
DE   AltName: Full=H4MPT formyltransferase {ECO:0000255|HAMAP-Rule:MF_00579};
GN   Name=ffsA {ECO:0000255|HAMAP-Rule:MF_00579, ECO:0000303|PubMed:10376840};
GN   OrderedLocusNames=MCA2858;
OS   Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylococcus.
OX   NCBI_TaxID=243233;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX   PubMed=10376840; DOI=10.1099/13500872-145-5-1235;
RA   Stolyar S., Costello A.M., Peeples T.L., Lidstrom M.E.;
RT   "Role of multiple gene copies in particulate methane monooxygenase activity
RT   in the methane-oxidizing bacterium Methylococcus capsulatus Bath.";
RL   Microbiology 145:1235-1244(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX   PubMed=11160118; DOI=10.1128/jb.183.5.1810-1812.2001;
RA   Stolyar S., Franke M., Lidstrom M.E.;
RT   "Expression of individual copies of Methylococcus capsulatus bath
RT   particulate methane monooxygenase genes.";
RL   J. Bacteriol. 183:1810-1812(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX   PubMed=15383840; DOI=10.1371/journal.pbio.0020303;
RA   Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S.,
RA   Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E.,
RA   Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J.,
RA   Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L.,
RA   Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H.,
RA   Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R.,
RA   Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A.;
RT   "Genomic insights into methanotrophy: the complete genome sequence of
RT   Methylococcus capsulatus (Bath).";
RL   PLoS Biol. 2:1616-1628(2004).
CC   -!- FUNCTION: Catalyzes the transfer of a formyl group from 5-formyl
CC       tetrahydromethanopterin (5-formyl-H(4)MPT) to methanofuran (MFR) to
CC       produce formylmethanofuran (formyl-MFR) and tetrahydromethanopterin
CC       (H(4)MPT). {ECO:0000255|HAMAP-Rule:MF_00579}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6,7,8-tetrahydromethanopterin + H(+) + N-formylmethanofuran
CC         = methanofuran + N(5)-formyl-5,6,7,8-tetrahydromethanopterin;
CC         Xref=Rhea:RHEA:18061, ChEBI:CHEBI:15378, ChEBI:CHEBI:57727,
CC         ChEBI:CHEBI:58018, ChEBI:CHEBI:58103, ChEBI:CHEBI:58151;
CC         EC=2.3.1.101; Evidence={ECO:0000255|HAMAP-Rule:MF_00579};
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (H(4)MPT route): step 4/5. {ECO:0000255|HAMAP-
CC       Rule:MF_00579}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00579}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00579}.
CC   -!- SIMILARITY: Belongs to the FTR family. {ECO:0000255|HAMAP-
CC       Rule:MF_00579}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U94337; AAK30007.1; -; Genomic_DNA.
DR   EMBL; AE017282; AAU91105.1; -; Genomic_DNA.
DR   RefSeq; WP_010962053.1; NC_002977.6.
DR   AlphaFoldDB; Q9ADT1; -.
DR   SMR; Q9ADT1; -.
DR   STRING; 243233.MCA2858; -.
DR   EnsemblBacteria; AAU91105; AAU91105; MCA2858.
DR   KEGG; mca:MCA2858; -.
DR   eggNOG; COG2037; Bacteria.
DR   HOGENOM; CLU_081314_0_0_6; -.
DR   OMA; VIMCPAE; -.
DR   OrthoDB; 895502at2; -.
DR   UniPathway; UPA00562; UER00704.
DR   Proteomes; UP000006821; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030270; F:formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity; ISS:JCVI.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015947; P:methane metabolic process; ISS:JCVI.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.520; -; 2.
DR   HAMAP; MF_00579; FTR; 1.
DR   InterPro; IPR014053; ForMFR_H4MPT_ForTrfase.
DR   InterPro; IPR002770; ForMFR_H4MPT_ForTrfase_C.
DR   InterPro; IPR023447; ForMFR_H4MPT_ForTrfase_fd-like.
DR   InterPro; IPR022667; ForMFR_H4MPT_ForTrfase_N.
DR   Pfam; PF01913; FTR; 1.
DR   Pfam; PF02741; FTR_C; 1.
DR   PIRSF; PIRSF006414; Ftr_formyl_trnsf; 1.
DR   SUPFAM; SSF55112; SSF55112; 2.
DR   TIGRFAMs; TIGR03119; one_C_fhcD; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cytoplasm; One-carbon metabolism; Reference proteome;
KW   Transferase.
FT   CHAIN           1..298
FT                   /note="Formylmethanofuran--tetrahydromethanopterin
FT                   formyltransferase"
FT                   /id="PRO_0000138123"
FT   CONFLICT        12
FT                   /note="F -> S (in Ref. 1; AAK30007 and 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   298 AA;  31545 MW;  58455F5DD1113C12 CRC64;
     MIINGVHIDE TFAEAFPMRA TRVIVTAQNL KWAHHAAQAM TGFATSVIAC GCEAGIEREL
     DPAETPDGRP GVSALLFAMG GKGLAKQLET RAGQCVLTSP TSALFAGIVE GEQIPLGKNL
     RYFGDGFQIS KRIGGKRYWR IPVMDGEFLC QETTGMIKAV GGGNFLILAE SQPQALAACE
     AAIEAMRRIP NVIMPFPGGV VRSGSKVGSK YKTLPASTND AFCPTLKGQT RTELSPEIES
     VMEIVIDGLS DADIAKAMRA GIEAACGLGA ANGIRRISAG NYGGKLGPFL FHLREIMA