ID   FTSH2_ORYSJ             Reviewed;         676 AA.
AC   Q655S1; A0A0P0X054; Q655S0;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=ATP-dependent zinc metalloprotease FTSH 2, chloroplastic;
DE            Short=OsFTSH2;
DE            EC=3.4.24.-;
DE   Flags: Precursor;
GN   Name=FTSH2; OrderedLocusNames=Os06g0669400, LOC_Os06g45820;
GN   ORFNames=OsJ_021445, P0686E06.31-1, P0686E06.31-2;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=16040665; DOI=10.1104/pp.105.061234;
RA   Yu F., Park S., Rodermel S.R.;
RT   "Functional redundancy of AtFtsH metalloproteases in thylakoid membrane
RT   complexes.";
RL   Plant Physiol. 138:1957-1966(2005).
CC   -!- FUNCTION: Probable ATP-dependent zinc metallopeptidase. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000250}; Single-pass membrane protein {ECO:0000250}; Stromal side
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q655S1-1; Sequence=Displayed;
CC   -!- SIMILARITY: In the N-terminal section; belongs to the AAA ATPase
CC       family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the peptidase M41
CC       family. {ECO:0000305}.
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DR   EMBL; AP003635; BAD45446.1; -; Genomic_DNA.
DR   EMBL; AP003635; BAD45447.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS99062.1; -; Genomic_DNA.
DR   EMBL; CM000143; EAZ37962.1; -; Genomic_DNA.
DR   RefSeq; XP_015643053.1; XM_015787567.1. [Q655S1-1]
DR   AlphaFoldDB; Q655S1; -.
DR   SMR; Q655S1; -.
DR   STRING; 4530.OS06T0669400-01; -.
DR   MEROPS; M41.005; -.
DR   PaxDb; Q655S1; -.
DR   PRIDE; Q655S1; -.
DR   EnsemblPlants; Os06t0669400-01; Os06t0669400-01; Os06g0669400. [Q655S1-1]
DR   EnsemblPlants; Os06t0669400-02; Os06t0669400-02; Os06g0669400. [Q655S1-1]
DR   GeneID; 4341796; -.
DR   Gramene; Os06t0669400-01; Os06t0669400-01; Os06g0669400. [Q655S1-1]
DR   Gramene; Os06t0669400-02; Os06t0669400-02; Os06g0669400. [Q655S1-1]
DR   KEGG; osa:4341796; -.
DR   eggNOG; KOG0731; Eukaryota.
DR   HOGENOM; CLU_000688_16_2_1; -.
DR   InParanoid; Q655S1; -.
DR   OMA; TLCPGHD; -.
DR   OrthoDB; 217929at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000007752; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   ExpressionAtlas; Q655S1; baseline and differential.
DR   Genevisible; Q655S1; OS.
DR   GO; GO:0009534; C:chloroplast thylakoid; IBA:GO_Central.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0004176; F:ATP-dependent peptidase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   Gene3D; 1.20.58.760; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01458; FtsH; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR041569; AAA_lid_3.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR003960; ATPase_AAA_CS.
DR   InterPro; IPR005936; FtsH.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000642; Peptidase_M41.
DR   InterPro; IPR037219; Peptidase_M41-like.
DR   Pfam; PF00004; AAA; 1.
DR   Pfam; PF17862; AAA_lid_3; 1.
DR   Pfam; PF01434; Peptidase_M41; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF140990; SSF140990; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01241; FtsH_fam; 1.
DR   PROSITE; PS00674; AAA; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; ATP-binding; Chloroplast; Hydrolase; Membrane;
KW   Metal-binding; Metalloprotease; Nucleotide-binding; Plastid; Protease;
KW   Reference proteome; Thylakoid; Transit peptide; Transmembrane;
KW   Transmembrane helix; Zinc.
FT   TRANSIT         1..32
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   TRANSIT         33..?
FT                   /note="Thylakoid"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..676
FT                   /note="ATP-dependent zinc metalloprotease FTSH 2,
FT                   chloroplastic"
FT                   /id="PRO_0000341338"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        476
FT                   /evidence="ECO:0000250"
FT   BINDING         254..261
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         475
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         479
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         553
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   676 AA;  72538 MW;  806A8A3623B4014D CRC64;
     MAPTSMSLAA KTPLPFSTLP SSGVAQRPVS VTASLEHKTN DARRKFLKLA LGNLGVGLPT
     LLGAKRALAE EQGVSSSRMS YSRFLEYLDK DRVKKVDLFE NGTIAIVEAI SPELGNRVQR
     VRVQLPGLSQ ELLQKLREKN IDFAAHSNQE DSGSLLFNLI GNLAFPLILI GGLFLLSRRA
     QGGLGGPNGP GFPLGFGQSR AKFQMEPNTG VTFDDVAGVD EAKQDFMEVV EFLKKPERFT
     AVGARIPKGV LLVGPPGTGK TLLAKAIAGE AGVPFFSISG SEFVEMFVGV GASRVRDLFK
     KAKENAPCIV FVDEIDAVGR QRGTGIGGGN DEREQTLNQL LTEMDGFEGN TGIIVIAATN
     RADILDSALL RPGRFDRQVS VDVPDVRGRT EILKVHGSNK KFDTDVSLEV IAMRTPGFSG
     ADLANLLNEA AILAGRRGRT AISSKEIDDS IDRIVAGMEG TVMTDGKSKS LVAYHEVGHA
     ICGTLTPGHD PVQKVTLIPR GQARGLTWFI PMDDPTLISR QQLFARIVGG LGGRAAEEII
     FGEPEVTTGA AGDLQQITGL AKQMVVTFGM SDIGPWSLMD SGAQSGDVIM RMMARNSMSE
     KLAEDIDTAV KRLSDEAYEI ALSQIRSNRE AMDKIVEVLL EKETLSGDEF RAILSEFTEI
     PVENRVPPAT PAALPA